Recombinant Human ENPP-7/Alk-SMase Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4924-EN
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human ENPP-7/Alk-SMase protein
Ala22-Ser439, with a C-terminal 10-His tag
Ala22-Ser439, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala22
Predicted Molecular Mass
49 kDa
SDS-PAGE
60-70 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze sphingomyelin to ceramide and phosphorylcholine. The phosphorylcholine is cleaved by Recombinant Human Alkaline Phosphatase/ALPL (Catalog # 2909-AP) and the phosphate is detected/measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996).
The specific activity is >14,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >14,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
4924-EN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: ENPP-7/Alk-SMase
ENPP-7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (alk-SMase) is expressed in the intestines and in human bile (1). It shares 30%‑36% homology with the members of the nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharing no similarities with neutral or acid SMase (2). Its main function is the digestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramide and phosphorylcholine. ENPP-7 is reported to hydrolyse and inactivate platelet-activating factor (PAF) by a phospholipase C-type activity (3). Studies also show a decrease in ENPP-7 activity in human colorectal adenocarcinomas and human colorectal carcinomas, which indicate a potential role of ENPP-7 in human colon cancer (4, 5).
References
- Duan, R-D. et al. (2006) Biochim. Biophys. 1761:281.
- Duan, R-D. et al. (2003) J. Biol. Chem. 278:38528.
- Wu, J. et al. (2006) Biochem J. 394:299.
- Hertervig, E. et al. (1996) Cancer. 79:448.
- Hertervig, E. et al. (1999) Br. J. Cancer. 81:232.
Long Name
Ectonucleotide Pyrophosphatase/Phosphodiesterase 7
Alternate Names
Alk-SMase, ENPP7, NPP7
Gene Symbol
ENPP7
UniProt
Additional ENPP-7/Alk-SMase Products
Product Documents for Recombinant Human ENPP-7/Alk-SMase Protein, CF
Product Specific Notices for Recombinant Human ENPP-7/Alk-SMase Protein, CF
For research use only
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