Recombinant Human Ephrin-B2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7397-EB

R&D Systems, part of Bio-Techne
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7397-EB-050
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Key Product Details

Source

NS0

Accession #

P52799

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Ephrin-B2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Ephrin-B2 protein
Human Ephrin-B2
(Met1-Ala229)
Accession # P52799		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys26

Predicted Molecular Mass

48.8 kDa (monomer)

SDS-PAGE

58-66 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human EphB2 Fc Chimera (Catalog # 5189-B2) is coated at 2 μg/mL, Recombinant Human Ephrin‑B2 Fc Chimera binds with an apparent Kd <0.1 nM.
Also measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neuron.

Formulation, Preparation and Storage

7397-EB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 300 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-B2

Ephrin-B2, also known as Htk-L, ELF-2, LERK-5, and NLERK-1, is a 40 kDa member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. The extracellular domains of Ephrin-B ligands are structurally related to GPI-anchored Ephrin-A ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. Ephrin-B2 preferentially interacts with receptors in the EphB family (1, 2). Mature human Ephrin-B2 consists of a 202 amino acid (aa) extracelluar domain (ECD), a 21 aa transmembrane segment, and an 83 aa cytoplasmic domain (3). Within the ECD, human Ephrin-B2 shares 97% aa sequence identity with mouse and rat Ephrin-B2. Ephrin-B2 is expressed presynaptically on neurons (4, 5). It promotes presynaptic development, EphB2 shedding, axonal growth cone collapse, and neurite repulsion, and also regulates inflammatory and neuropathic pain (4-6). Ephrin-B2 is expressed by vascular mural cells and arterial vascular and lymphatic endothelium (7, 8). It exerts proliferative and migratory effects on these cells during angiogenesis and lymphangiogenesis in part by regulating the signaling activity of VEGF R2 and VEGF R3 (7-9). Ephrin-B2 plays a role in the immune response by mediating monocyte extravasation and T cell costimulation (10, 11). It is up‑regulated in invasive cancers and promotes tumor cell migration, invasion, and tumor angiogenesis (12-14). It functions as a cellular entry receptor for Hendra and Nipah viruses (15). Ephrin-B2 is also important for the separation of the urinary and intestinal tracts during development (16).

References

  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Cerretti, D.P. et al. (1995) Mol. Immunol. 32:1197.
  4. McClelland, A.C. et al. (2009) Proc. Natl. Acad. Sci. 106:20487.
  5. Zhao, J. et al. (2010) Mol. Pain 6:77.
  6. Lin, K.-T. et al. (2008) J. Biol. Chem. 283:28969.
  7. Foo, S.S. et al. (2006) Cell 124:161.
  8. Wang, Y. et al. (2010) Nature 465:483.
  9. Sawamiphak, S. et al. (2010) Nature 465:487.
  10. Pfaff, D. et al. (2008) J. Cell Sci. 121:3842.
  11. Yu, G. et al. (2003) J. Immunol. 171:106.
  12. Meyer, S. et al. (2005) Int. J. Oncol. 27:1197.
  13. Nakada, M. et al. (2010) Int. J. Cancer 126:1155.
  14. Liu, W. et al. (2004) Br. J. Cancer 90:1620.
  15. Bonaparte, M.I. et al. (2005) Proc. Natl. Acad. Sci. 102:10652.
  16. Dravis, C. et al. (2004) Dev. Biol. 271:272.

Alternate Names

EFNB2, ELF-2, EphrinB2, Htk-L, LERK-5, NLERK-1

Entrez Gene IDs

1948 (Human); 13642 (Mouse); 30219 (Zebrafish)

Gene Symbol

EFNB2

UniProt

P52799

Additional Ephrin-B2 Products

Product Documents for Recombinant Human Ephrin-B2 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Ephrin-B2 Fc Chimera Protein, CF

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