Recombinant Human ErbB3/Her3 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10701

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

P21860.1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all ErbB3/Her3 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human ErbB3/Her3 protein
Human ErbB3/Her3
(Ser20-Thr643)
Accession # P21860.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser20

Predicted Molecular Mass

97 kDa

SDS-PAGE

110-130 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein (Catalog # 377-HB/CF) is coated at 1 µg/mL (100 μL/well), Biotinylated Recombinant Human ErbB3/Her3 Fc Chimera Avi-tag (Catalog # AVI10701) binds with an ED50 of 0.75-6 ng/mL.

Scientific Data Images for Recombinant Human ErbB3/Her3 Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human ErbB3/Her3 Fc Chimera Avi-tag Protein Binding Activity.

When Recombinant Human NRG1-beta 1/HRG1-beta 1 ECD Protein (377-HB/CF) is coated at 1 µg/mL, Biotinylated Recombinant Human ErbB3/Her3 Fc Chimera Avi-tag (Catalog # AVI10701) binds with an ED50 of 0.75-6 ng/mL.

Biotinylated Recombinant Human ErbB3/Her3 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human ErbB3/Her3 Fc Chimera Avi-tag (Catalog # AVI10701) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-130 kDa and 220-260 kDa, respectively.

Formulation, Preparation and Storage

AVI10701
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ErbB3/Her3

ErbB3, also called human epidermal growth factor receptor 3 (HER3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors (1). Human ErbB3 consists of an extracellular domain (ECD) with four, ligand binding subdomains, I-IV, a transmembrane domain and a cytoplasmic region with a defective kinase domain unique among ErbB family members (1-3). Additionally, the cytoplasmic region contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85 (1-3). The mature ECD of human ErbB3 shares 92% amino acid sequence identity with the ECD of mouse and rat ErbB3. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation (4, 5). Monomeric ErbB3 serves as a low affinity receptor for the heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex (6, 7). Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor (8). It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2 (9). ErbB3 overexpression in various solid tumor is associated with shorter survival of patients and, subsequently, ErbB3 signaling is a target for cancer therapy. Current clinical results suggested that ErbB3 therapies may have the most efficacy in combination therapy (10). Biotinylated Recombinant Human ErbB3 Fc Chimera Avi-tag protein has high binding affinity to Recombinant Human NRG1-beta 1. Our Avi-tag Biotinylated ErbB3 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation.

References

  1. Wieduwilt, M.J. and Moasser, M.M. (2008) Cell Mol Life Sci. 65:1566.
  2. Sithanandam, G. and Anderson, L.M. (2008) Cancer Gene Therapy 15:413.
  3. Sierke, S.L. et al. (1997) The Biochemical journal 322:757.
  4. Campbell, M.R. et al. (2010) Clin Cancer Res 16:1373.
  5. Yarden, Y. and Sliwkowski, M.X. (2001) Nat Rev Mol Cell Biol 2:127.
  6. Graus-Porta, D. et al. (1997), EMBO J. 1997 16:1647.
  7. Hellyer, N.J. et al. (2001) J. Biol. Chem. 276:42153.
  8. Linggi, B. and Carpenter, G. (2006) Trends Cell Biol. 16:649.
  9. Schaefer, G. et al. (1999) J. Biol. Chem. 274:859.
  10. Hafeez, U. et al. (2020) Expert Rev. Anticancer Ther. 20:1057.

Long Name

Receptor Tyrosine Protein Kinase ErbB3

Alternate Names

HER3

Entrez Gene IDs

2065 (Human); 13867 (Mouse)

Gene Symbol

ERBB3

UniProt

P21860.1

Additional ErbB3/Her3 Products

Product Documents for Recombinant Human ErbB3/Her3 Fc Chimera Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human ErbB3/Her3 Fc Chimera Avi-tag Protein, CF

For research use only

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