Recombinant Human FABP1/L-FABP Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9465-CL

R&D Systems, part of Bio-Techne
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9465-CL-100
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Key Product Details

Source

E. coli

Accession #

AAA52418

Conjugate

Unconjugated

View all FABP1/L-FABP Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human FABP1/L-FABP protein
Ser2-Ile127, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2

Predicted Molecular Mass

15 kDa

SDS-PAGE

14 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9465-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
Reconstitution
Reconstitute at 1 mg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP1/L-FABP

Fatty acid binding protein-1 (FABP1; also named L- or liver FABP, hepatic FABP, Z protein, and heme-binding protein) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner (1, 10, 11). FABP1 is one of ten cytoplasmic FABPs that are 14-15 kDa in size and range from 126-140 amino acids (aa) in length (1, 2, 3). Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. The FABP family members are subdivided based on organ or tissue type it was originally expressed or identified; liver- (L-FABP), intestine- (I-FABP), heart- (H-FABP), adipocyte- (A-FABP), epidermal- (E-FABP), ileal- (IL-FABP), brain- (B-FABP), myelin- (M-FABP) and testis-FABP (T-FABP) (1). Human L-FABP, the product of the FABP1 gene, is a 127 aa cytosolic protein that shows a flattened beta -barrel structure generated by a series of antiparallel beta -strands and two alpha ‑helices (4, 7). Unlike other members in the FABP family, each molecule of FABP1 is capable of binding two molecules of long-chain fatty acids. Other ligands may include heme, steroids, acyl CoAs, oxidized/peroxidized fatty acids, leukotrienes, prostaglandins and peroxisome proliferators (5, 6, 7, 13). It is suggested that ligands first bind to the outside of the molecule, and this binding subsequently induces a conformational change in the binding protein, resulting in "internalization" of the ligand (7, 12). An Ala-to-Thr polymorphism at position # 54 has been associated with an increased risk of type II diabetes (7, 10) and a Thr-to-Ala polymorphism at position # 94 has been reported to increase cholesterol binding affinity (8). Human FABP1 is 84%, 82% and 90% aa identical to mouse, rat and canine FABP1, respectively (9). It also shows 29% and 24% aa identity to human H-FABP and I‑FABP, respectively.

References

  1. Smathers, R & Petersen, D. (2011) Human Genomics 5:170.
  2. Storch, J. & Thumser, AE. (2000) Biochim Biophys Acta. 1486:28.
  3. Mihajlovic,M. & Lazaridis, T. (2007) Protein Sci. 9:2042.
  4. Sweetser, D.A. et al. (1987) J. Biol. Chem. 262:16060.
  5. Wang, G. et al. (2015) J Lipid Res. 56:2238.
  6. Thompson, J. et al. (1997) J. Biol. Chem. 272:7140.
  7. Bernlohr, DA. et al. (1997) Ann. Rev. of Nut. 17:277.
  8. Huang, H. et al. (2015) Biochim Biophys Acta. 1851:946
  9. Lowe J.B. et al. (1985) J. Biol. Chem. 260:3413.
  10. Zimmerman, A.W. and J.H. Veerkamp (2002) Cell. Mol. Life Sci. 59:1096.
  11. Haunerland, N.H. and F. Spener (2004) Prog. Lipid Res. 43:328.
  12. Majava,V. et al. (2010) PLoS One. 5:e10300.
  13. Veerkamp JH, Maatman RGHJ. (1995) Prog. Lipid Res. 34:17.

Long Name

Fatty Acid-Binding Protein 1

Alternate Names

L-FABP, LFABP

Entrez Gene IDs

2168 (Human); 14080 (Mouse); 24360 (Rat)

Gene Symbol

FABP1

UniProt

AAA52418

Additional FABP1/L-FABP Products

Product Documents for Recombinant Human FABP1/L-FABP Protein, CF

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Product Specific Notices for Recombinant Human FABP1/L-FABP Protein, CF

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