Recombinant Human FABP3/H-FABP Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9479-CL

R&D Systems, part of Bio-Techne
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Key Product Details

Source

E. coli

Accession #

P05413

Conjugate

Unconjugated

View all FABP3/H-FABP Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human FABP3/H-FABP protein
MHHHHHH GS  Human FABP3/H-FABP
(Met1-Ala133)
Accession # P05413
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

16 kDa

SDS-PAGE

15 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9479-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and DTT.
Reconstitution
Reconstitute at 1 mg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FABP3/H-FABP

Fatty acid binding protein-3 (FABP3; also named Fatty acid binding protein 11, FABP11, H- or M- (heart or muscle) FABP, Mammary derived growth inhibitor, or MDGI) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner (1, 9, 10). FABP3 is one of ten cytoplasmic FABPs that are 14-15 kDa in size and range from 126-140 amino acids (aa) in length (1, 2, 3). Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. The FABP family members are subdivided based on organ or tissue type it was originally expressed or identified; liver- (L-FABP), intestine- (I-FABP), heart- (H-FABP), adipocyte- (A-FABP), epidermal- (E-FABP), ileal- (IL-FABP), brain- (B-FABP), myelin- (M-FABP) and testis-FABP (T-FABP) (1). Human H-FABP, the product of the FABP3 gene, is a 132 aa cytosolic protein that shows a flattened beta -barrel structure generated by a series of antiparallel beta -strands and two alpha ‑helices (4, 5, 7). One molecule of FABP3 is capable of binding one long-chain fatty acid (6, 8, 11). It is suggested that ligands first bind to the outside of the molecule, and this binding subsequently induces a conformational change in the binding protein, resulting in "internalization" of the ligand (7, 11). Human FABP3 is 86%, 89% and 89% aa identical to mouse, rat and canine FABP3, respectively. It also shows 29% and 32% aa identity to human L-FABP and I-FABP, respectively.

References

  1. Smathers, R & Petersen, D. (2011) Human Genomics 5:170.
  2. Storch, J. & Thumser, AE. (2000) Biochim Biophys Acta. 1486:28.
  3. Mihajlovic, M. & Lazaridis, T. (2007) Protein Sci. 9:2042.
  4. Sweetser, D.A. et al. (1987) J. Biol. Chem. 262:16060.
  5. Offner, G. et al. Biochem. J. (1988) 252:191.
  6. Glatz JF. et al. (1985) Chem Phys Lipids. 38:115.
  7. Bernlohr, DA. et al. (1997) Ann. Rev. of Nut. 17:277.
  8. Zanotti G. et al. (1992) J. Biol. Chem. 267:18541.
  9. Zimmerman, A.W. & J.H. Veerkamp (2002) Cell. Mol. Life Sci. 59:1096.
  10. Haunerland, N.H. and F. Spener (2004) Prog. Lipid Res. 43:328.
  11. Majava,V. et al. (2010) PLoS One. 5:e10300.

Long Name

Fatty Acid-Binding Protein 3

Alternate Names

H-FABP, HFABP, MDGI

Entrez Gene IDs

2170 (Human)

Gene Symbol

FABP3

UniProt

P05413

Additional FABP3/H-FABP Products

Product Documents for Recombinant Human FABP3/H-FABP Protein, CF

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Product Specific Notices for Recombinant Human FABP3/H-FABP Protein, CF

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