Recombinant Human Fc gamma RIIIB/CD16b His Avi Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11507

His-tag Avi-tag
R&D Systems, part of Bio-Techne
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AVI11507-050
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Key Product Details

Source

CHO

Accession #

O75015.2

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all Fc gamma RIIIB/CD16b Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Fc gamma RIIIB/CD16b protein
Human FCGRIIIB
(Gly17-Gln208)
Accession # O75015.2		 6-His tag Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly17

Predicted Molecular Mass

24 kDa

SDS-PAGE

39-63 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human Fc gamma RIIIB/CD16b His-tag Avi-tag (Catalog # AVI11507) binds Human IgG with an ED50 of 60.0-720 ng/mL.

Scientific Data Images for Recombinant Human Fc gamma RIIIB/CD16b His Avi Protein, CF

Recombinant Human Fc gamma RIIIB/CD16b His-tag Avi-tag Protein Binding Activity.

Recombinant Human Fc gamma RIIIB/CD16b His-tag Avi-tag Protein (Catalog # AVI11507) binds Human IgG with an ED50 of 60.0-720 ng/mL.

Recombinant Human Fc gamma RIIIB/CD16b His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human Fc gamma RIIIB/CD16b His-tag Avi-tag Protein (Catalog # AVI11507) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 39-63 kDa, under reducing conditions.

Formulation, Preparation and Storage

AVI11507
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIIB/CD16b

Receptors for the Fc region of IgG (Fc gamma R) are members of the Ig superfamily. Based on the genetic organization and molecular structure of receptors for the Fc region of IgG (Fc gamma R), three classes of human Fc gamma Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized (1-3). These receptors function in the activation or inhibition of immune responses. The activating-type receptor either has, or associates non-covalently with an accessory subunit (FcR gamma or zeta chain) that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. In contrast, the inhibitory receptor (Fc gamma RIIB) has a built-in immunoreceptor tyrosine-based inhibitory motif (ITIM) in its own cytoplasmic domain. Fc gamma RI is a high‑affinity receptor that binds monomeric IgG. Both Fc gamma RII and RIII are low-affinity receptors that bind IgG in the form of immune complexes. Two genes for human Fc gamma RIII, A and B, encoding a transmembrane receptor and a glycosylphosphatidylinositol (GPI) anchored protein, respectively, have been identified. Three allelic variants of Fc gamma RIIIB, NA-1, NA-2, and SH, exist. A soluble form of Fc gamma RIIIB corresponding to the extracellular region of the receptor is produced by proteolytic cleavage and circulates in plasma and other body fluids. The extracellular region of Fc gamma RIIIB share 62% and 59% sequence homology with the mouse and rat variants, respectively. The extracellular domains of human Fc gamma RIIIA and B share 97% amino acid sequence homology. Whereas Fc gamma RIIIA is expressed on most effector cells of the immune system including macrophage, monocyte, NK cells, mast cells, eosinophils, dendritic cells and Langerhans cells, Fc gamma RIIIB is selectively expressed in neutrophils and eosinophils. Signaling through Fc gamma RIIIA results in oxidative burst, cytokine release and phagocytosis by macrophages, antibody-dependent cellular cytotoxicity by natural killer cells and degranulation of mast cells. By contrast, Fc gamma RIIIB is a decoy receptor that binds IgG complexes without triggering activation. Soluble Fc gamma RIIIB has a regulatory role in inflammatory processes (4). It interacts with complement receptors CR3 and CR4 on monocytes to induce the production of pro-inflammatory cytokines.  Our Avi-tag Biotinylated Human Fc gamma RIIIB His-tag features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
  2. Ravetch, J.V. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Gauchat, G.J. et al. (1996) J. Immunol. 157:1184.

Long Name

Fc gamma Receptor III

Alternate Names

CD16b, FCG3, FCGR3B, FcgRIIIB, FcR-10, FCRIIIB, IGFR3

Entrez Gene IDs

2215 (Human); 14131 (Mouse)

Gene Symbol

FCGR3B

UniProt

O75015.2

Additional Fc gamma RIIIB/CD16b Products

Product Documents for Recombinant Human Fc gamma RIIIB/CD16b His Avi Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Fc gamma RIIIB/CD16b His Avi Protein, CF

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