Recombinant Human FCRL4/FcRH4 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2426-FC

R&D Systems, part of Bio-Techne
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2426-FC-050
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Key Product Details

Source

CHO

Accession #

Q96PJ5

Conjugate

Unconjugated

Applications

Bioactivity

View all FCRL4/FcRH4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human FCRL4/FcRH4 protein
Ala18-Arg385, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala18

Predicted Molecular Mass

42 kDa

SDS-PAGE

53-60 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human FCRL4/FcRH4 is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Human IgA binds with an ED50 of 5-20 μg/mL.

Reviewed Applications

Read 3 reviews rated 5 using 2426-FC in the following applications:

Scientific Data Images for Recombinant Human FCRL4/FcRH4 His-tag Protein, CF

Recombinant Human FCRL4/FcRH4 His-tag Protein Binding Activity

Recombinant Human FCRL4/FcRH4 His-tag Protein Binding Activity

When Recombinant Human FCRL4 (Catalog # 2426-FC) is coated onto a microplate at 0.5 µg/mL, Biotinylated Human IgA binds with an ED50 of 5-20 μg/mL.
Recombinant Human FCRL4/FcRH4 His-tag Protein SDS-PAGE

Recombinant Human FCRL4/FcRH4 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human FCRL4/FcRH4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 53-60 kDa and 45-52 kDa, respectively.

Formulation, Preparation and Storage

2426-FC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: FCRL4/FcRH4

Fc receptor-like 4 (FCRL4), also known as FcRH4, IFGP2 and IRTA1, is a member of the Ig superfamily transmembrane protein, which is preferentially expressed in B cells and contains immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (1). Mature human FCRL4 consists of a 368 amino acid (aa) extracellular domain (ECD) with four Ig-like C2-type domains, a 21 aa transmembrane segment, and a 107 aa cytoplasmic domain with three ITIM-like motifs. FCRL4 is closely related to FC gammaRIIB (2, 3) and shows high homology to Fc receptors (4). In cellular binding assays, FCRL4 bound to IgA efficiently (5). The FCRL4 gene is localized in the human chromosome 1q21-23 region, a hotspot for translocation events (3). The B cells expressing FCRL4 participate in the autoimmune response in rheumatoid arthritis patients (6).

References

  1. Ehrhardt, G.R. et al. (2003) Proc. Natl. Acad. Sci. U.S.A. 100:13489.
  2. Davis, R.S. et al. (2001) Proc. Natl. Acad. Sci. U. S. A. 98:9772.
  3. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
  4. Amara, K. et al. (2007) J. Autoimmun. 81:34.
  5. Wilson, T.J. et al. (2012) J. Immunol. 188:4741.
  6. Miller, I. et al. (2002) Blood 99:2662.

Long Name

Fc Receptor-like protein 4

Alternate Names

CD307d, FcRH4, IGFP2, IRTA1

Entrez Gene IDs

83417 (Human)

Gene Symbol

FCRL4

UniProt

Q96PJ5

Additional FCRL4/FcRH4 Products

Product Documents for Recombinant Human FCRL4/FcRH4 His-tag Protein, CF

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Product Specific Notices for Recombinant Human FCRL4/FcRH4 His-tag Protein, CF

For research use only

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