Recombinant Human Fetuin B Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1725-PI
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Fetuin B protein
Met19-Pro382, with a C-terminal 10-His tag
Met19-Pro382, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met19
Predicted Molecular Mass
42 kDa
SDS-PAGE
54 kDa and 58 kDa, reducing conditions
Activity
Measured by its ability to inhibit active Cathepsin V cleavage of a fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008).
The IC50 value is <150 nM, as measured under the described conditions.
The IC50 value is <150 nM, as measured under the described conditions.
Formulation, Preparation and Storage
1725-PI
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Fetuin B
Fetuins are members of the cystatin superfamily of cysteine protease inhibitors (1-3). Additional members of this superfamily are kininogen and histidine-rich glycoprotein. Fetuin A and B are two known members of the fetuin family. Hepatocytes are believed to be the principal cellular source, but other cell types also express it (4, 5). Fetuin A, also known as alpha2-Heremans-Schmid glycoprotein, is an inhibitor of basic calcium phosphate precipitation and a negative acute-phase protein (6, 7). Normal circulating levels of Fetuin A in adults (300-600 μg/mL) fall significantly (30-50%) during injury and infection (7). Fetuin B is a newer member whose function is not fully characterized (1, 2). Fetuin A and B display similarities and differences in their characteristics. Fetuin B exhibits reduction of calcification, while both mRNA levels were down‑regulated during the acute phase in inflammation-induced rats (4). However, they share only 20% amino acid sequence identity (2). The amounts of Fetuin B in human serum, unlike Fetuin A, vary with gender and are higher in females than in males (4).
References
- Oliver, E., et al. (1999) Genomics. 57:352.
- Oliver, E., et al. (2000) Biochem. J. 350:589.
- Kellemann, J. et al., 1989, J. Biol. Chem. 264:14121.
- Denecke, B. et al. (2003) Biochem. J. 376:135.
- Schäfer, C., et al. (2003) J. Clin. Invest. 112:357.
- Dziegielewska, K. M., et al. (1996) Histochem. Cell Biol. 106:319.
- Gangneux, C. et al. (2003) Nucleic Acids Res. 31:5957.
Alternate Names
FETUB
Entrez Gene IDs
26998 (Human)
Gene Symbol
FETUB
UniProt
Additional Fetuin B Products
Product Documents for Recombinant Human Fetuin B Protein, CF
Product Specific Notices for Recombinant Human Fetuin B Protein, CF
For research use only
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