Recombinant Human FGF-10 Protein Best Seller

R&D Systems, part of Bio-Techne | Catalog # 345-FG

R&D Systems, part of Bio-Techne
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Carrier Free
345-FG-025/CF
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With Carrier
345-FG-025
345-FG-250
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Key Product Details

Source

E. coli

Accession #

O15520

Conjugate

Unconjugated

Applications

Bioactivity

View all FGF-10 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human FGF-10 protein
Cys37-Ser208 & Gly41-Ser208

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys37 & Gly41

Predicted Molecular Mass

19.5 kDa

SDS-PAGE

19-22 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802.
The ED50 for this effect is 20-100 ng/mL.

Reviewed Applications

Read 1 review rated 5 using 345-FG in the following applications:

Scientific Data Images for Recombinant Human FGF-10 Protein

Recombinant Human FGF-10 Protein Bioactivity

Recombinant Human FGF-10 Protein Bioactivity

Recombinant Human FGF-10 (Catalog # 345-FG) stimulates cell proliferation of the 4MBr‑5 rhesus monkey epithelial cell line. The ED50 for this effect is 20-100 ng/mL.
Recombinant Human FGF-10 Protein SDS-PAGE

Recombinant Human FGF-10 Protein SDS-PAGE

1 μg/lane of Recombinant Human FGF-10 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 21 kDa.

Adult stem cell-derived Lung Organoids Cultured using Recombinant Human FGF-10 Protein.

Adult stem cells isolated from human lung biopsy tissue were embedded in Cultrex UltiMatrix RGF Basement Membrane Extract (BME001-05) and cultured for 20-60 days in lung organoid expansion medium, which includes Recombinant Human FGF-10 (Catalog # 345-FG), Recombinant Human KGF/FGF-7 (251-KG), Recombinant Human Noggin (6057-NG), and Recombinant Human R-Spondin 1 (4645-RS), along with the other reagents listed in the lung organoid expansion medium recipe in the lung organoid culture protocol. Lung organoids were able to differentiate and exhibit markers for various cell types of the lung. Lung organoids were stained with (A) a Rabbit Anti-Human Cytokeratin 5 (KRT5) Monoclonal Antibody (Novus Biologicals, Catalog # NB110-56916; green) and a Goat Anti-Human p63/TP73L Polyclonal Antibody (AF1916; red) to visualize basal cells, (B) a Hamster Anti-Mouse Podoplanin (PDPN) Monoclonal Antibody (Novus Biologicals, Catalog # NB600-1015; green) to visualize alveolar type I cells and a Goat Anti-Human p63/TP73L Polyclonal Antibody (AF1916; red) to visualize basal cells, and (C, D) a Mouse Anti-MUC5AC Monoclonal Antibody (Novus Biologicals, Catalog # NBP2-15196; green) to visualize Goblet cells and a Mouse Anti-Human/Mouse/Rat SOX2 Monoclonal Antibody (MAB2018; red). All samples were counterstained with DAPI (5748; blue).

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 345-FG
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 345-FG/CF
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-10

The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP (1, 4). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (5). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation (6), palate development (7), branching morphogenesis and directional outgrowth of lung buds (8, 9), formation of the otic vesicle and chochlea (10), adipogenesis (11), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (12 - 15). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (7, 10, 13 ‑ 15). The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung (8, 9). Overlapping expression patterns and activities with FGF-3, -7,  and -8 suggest at least a partial redundancy in FGF‑10 biology (7, 10, 14, 15). FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer (16).

References

  1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
  2. Igarashi, M. et al. (1998) J. Biol. Chem. 273:13230.
  3. Emoto, H. et al. (1997) J. Biol. Chem. 272:23191.
  4. Beer, H.-D. et al. (2005) Oncogene 24:5269.
  5. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  6. Min, H. et al. (1998) Genes Dev. 12:3156.
  7. Rice, R. et al. (2004) J. Clin. Invest. 113:1692.
  8. Bellusci, S. et al. (1997) Development 124:4867.
  9. Weaver, M. et al. (2000) Development 127:2695.
  10. Pirvola, U. et al. (2000) J. Neurosci. 20:6125.
  11. Sakaue, H. et al. (2002) Genes Dev. 16:908.
  12. Donjacour, A.A. et al. (2003) Dev. Biol. 261:39.
  13. Mailleux, A.A. et al. (2002) Development 129:53.
  14. Makarenkova, H.P. et al. (2000) Development 127:2563.
  15. Jaskoll, T. et al. (2005) BMC Dev. Biol. 5:11.
  16. Nomura, S. et al. (2008) Br. J. Cancer 99:305.

Long Name

Fibroblast Growth Factor 10

Alternate Names

FGF10, KGF-2, KGF2

Entrez Gene IDs

2255 (Human); 14165 (Mouse); 25443 (Rat)

Gene Symbol

FGF10

UniProt

O15520

Additional FGF-10 Products

Product Documents for Recombinant Human FGF-10 Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human FGF-10 Protein

For research use only

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