Recombinant Human Fibulin 1C Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9007-FB

R&D Systems, part of Bio-Techne
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9007-FB-050
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Key Product Details

Source

HEK293

Accession #

NP_001987

Conjugate

Unconjugated

Applications

Bioactivity

View all Fibulin 1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Fibulin 1 protein
Asp30-Leu683, with an N-terminal HA tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr

Predicted Molecular Mass

73 kDa

SDS-PAGE

84-104 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of HFL1 human fetal lung fibroblast cells.
The ED50 for this effect is 0.7-3.5 μg/mL.

Formulation, Preparation and Storage

9007-FB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibulin 1

Fibulin 1 is a 95 kDa matricellular glycoprotein component of the elastic fiber core in connective tissues (1-3). It contains 3 tandem anaphylatoxin-like domains followed by 9 tandem EGF-like domains (4). Alternative splicing of human Fibulin 1 generates additional isoforms with a deletion or substitution of the C-terminal 137 amino acids (aa) (4). The Fibulin 1c isoform carries a 117 aa substitution for this region which includes a portion of the ninth EGF-like domain and the C-terminal region. Human Fibulin 1c shares 86% aa sequence identity with mouse and rat Fibulin 1c. It self-aggregates and additionally binds to several extracellular matrix and cellular proteins including Aggrecan, Versican, Fibronectin, ECM1, NOV/CCN3, and pro-HB-EGF (5-9). Fibulin 1c enhances ADAMTS-1 mediated cleavage of Aggrecan and Versican (10, 11) and can itself be cleaved by neutrophil elastase or MMP-13 (12, 13). It functions as a bridge between Fibrinogen and Fibrin, enabling Fibrin attachment to the platelet cell surface via Integrin  alpha2b beta3 (14). Fibulin 1 inhibits cardiomyocyte proliferation and Fibronectin-mediated cell adhesion and can promote bone formation (11, 15, 16).

References

  1. Zheng, P. et al. (2015) Pharmacol. Res. 99:11.
  2. Murphy-Ullrich, J.E. and E.H. Sage (2014) Matrix Biol. 37:1.
  3. Roark, E.F. et al. (1995) J. Histochem. Cytochem. 43:401.
  4. Argraves, W.S. et al. (1990) J. Cell Biol. 111:3155.
  5. Aspberg, A. et al. (1999) J. Biol. Chem. 274:20444.
  6. Balbona, K. et al. (1992) J. Biol. Chem. 267:20120.
  7. Fujimoto, N. et al. (2005) Biochem. Biophys. Res. Commun. 333:1327.
  8. Perbal, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:869.
  9. Brooke, J.S. et al. (2002) BMC Cell Biol. 3:2.
  10. Lee, N.V. et al. (2005) J. Biol. Chem. 280:34796.
  11. Cooley, M.A. et al. (2012) Dev. Dyn. 241:303.
  12. Sasaki, T. et al. (1996) Eur. J. Biochem. 240:427.
  13. Wang, Q. et al. (2015) Oncogene 34:1006.
  14. Godyna, S. et al. (1996) Blood 88:2569.
  15. Twal, W.O. et al. (2001) J. Cell Sci. 114:4587.
  16. Cooley, M.A. et al. (2014) Bone 69:30.

Alternate Names

FBLN1, FIBL1

Entrez Gene IDs

2192 (Human); 14114 (Mouse); 315191 (Rat)

Gene Symbol

FBLN1

UniProt

NP_001987

Additional Fibulin 1 Products

Product Documents for Recombinant Human Fibulin 1C Protein, CF

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Product Specific Notices for Recombinant Human Fibulin 1C Protein, CF

For research use only

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