Recombinant Human Fibulin 2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9559-FB

R&D Systems, part of Bio-Techne
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9559-FB-050
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Key Product Details

Source

HEK293

Accession #

P98095-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Fibulin 2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Fibulin 2 protein
Ala28-Leu1184 (Thr854Ala), with an N-terminal HA tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Tyr (of HA tag)

Predicted Molecular Mass

125 kDa

SDS-PAGE

150-175 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of HFL1 human fetal lung fibroblast cells.
The ED50 for this effect is 0.15-0.9 μg/mL.

Scientific Data Images for Recombinant Human Fibulin 2 Protein, CF

Recombinant Human Fibulin 2 Protein Bioactivity

Recombinant Human Fibulin 2 Protein Bioactivity

Recombinant Human Fibulin 2 (Catalog # 9559-FB) supports HFL1 human fetal lung fibroblast cell adhesion. The ED50 for this effect is 0.15-0.9 μg/mL

Formulation, Preparation and Storage

9559-FB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibulin 2

Fibulin 2 is the second largest member of the seven-member fibulin family of extracellular membrane (ECM) glycoproteins. Fibulin-2 consists of 1184 amino acids (aa) with a predicted molecular weight of ~125 kDa. The overall structure is common to fibulins: 3 Anaphylatoxin (AT)-like domains, 11 Epidermal Growth Factor (EGF)-like domains, and a Fibulin-type carboxy-terminal (FC) domain (1-4). Fibulin 2 is considered a Class I fibulin because the 400-residue N-terminus is divided into the Na and Nb sections, with the Na section containing 150-residues and 12 cysteines while the remaining Nb section is cysteine-free (2, 3). The protein is known to form disulfide-linked homodimers, but it can also be secreted as an oligomer (5). Fibulin 2 is highly conserved across species, with the human protein sharing 82% amino acid identity compared to both mouse and rat. Fibulin 2 is considered a multifunctional binding protein due to its association with numerous ECM components, but its specific interactions have yet to be determined (6). Fibulin 2 is localized at the interface between microfibrils and the elastin core and its known interactions include nidogen-1, perlecan, laminin, aggrecan, endostatins, versican, collagen, and tropoelastin (1, 2, 4, 7). It is down-regulated in numerous forms of cancer including breast, colorectal, lung, esophageal, and squamous cell carcinoma, but over-expression has been shown with solid tumors (1, 3, 4). There is also evidence that suggests that Fibulin 2 may play an indirect role in the neurogenesis of adult neural stem cells via interaction with integrins and TGF-beta 1 (8).

References

  1. Alcendor et al. (2011) Am. J. Pathol. 179:1443.
  2. Timpl et al. (2003) Nat. Rev. Mol. Cell Biol. 4:479.
  3. Kanan et al. (2014) J. Biol. Chem. 289:13419.
  4. Argraves et al. (2003) EMBO reports 4:1127.
  5. Pan et al. (1993) J. Cell Biol. 123:1269.
  6. Fontanil et al. (2014) Oncotarget 5:1253.
  7. El-Hallous et al. (2007) J. Biol. Chem. 282:8935.
  8. Radice et al. (2015) Mol. Cell. Neurosci. 67:75.

Alternate Names

FBLN2, FIBL-2, Fibulin-2

Entrez Gene IDs

2199 (Human); 14115 (Mouse); 282583 (Rat)

Gene Symbol

FBLN2

UniProt

P98095-1

Additional Fibulin 2 Products

Product Documents for Recombinant Human Fibulin 2 Protein, CF

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Product Specific Notices for Recombinant Human Fibulin 2 Protein, CF

For research use only

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