Recombinant Human Flt-3/Flk-2 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11064

Biotinylated
R&D Systems, part of Bio-Techne
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AVI11064-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P36888.2

Structure / Form

Disulfide-linked homodimer. Biotinylated via Avi-tag.

Conjugate

Biotin

Applications

Bioactivity

View all Flt-3/Flk-2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Flt-3/Flk-2 protein
Human FLT-3
(Asn27-Asn541)
Accession # P36888.2		 IEGRMD

Human IgG1
(Pro100-Lys330)

 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.50 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asn27

Predicted Molecular Mass

87 kDa

SDS-PAGE

105-125 kDa, under reducing conditions.

Activity

When Biotinylated Recombinant Human Flt-3/Flk-2 Fc Chimera Avi-tag (Catalog # AVI11064) is immobilized at 0.2 µg/mL (100 µL/well), Recombinant Human Flt-3 Ligand/FLT3L  (Catalog # 308-FK) binds with an ED50 of 0.060-0.720 ng/mL.

Scientific Data Images for Recombinant Human Flt-3/Flk-2 Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human Flt‑3/Flk‑2 Fc Chimera Avi-tag Protein Binding Activity.

When Biotinylated Recombinant Human Flt-3/Flk-2 Fc Chimera Avi-tag Protein (Catalog # AVI11064) is immobilized at 0.2 µg/mL (100 µL/well), Recombinant Human Flt-3 Ligand/FLT3L (308-FK) binds with an ED50 of 0.060-0.720 ng/mL.

Biotinylated Recombinant Human Flt‑3/Flk‑2 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human Flt‑3/Flk‑2 Fc Chimera Avi-tag Protein (Catalog # AVI11064) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 105-125 kDa and 210-250 kDa, respectively.

Formulation, Preparation and Storage

AVI11064
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Flt-3/Flk-2

FMS-like tyrosine kinase 3 (Flt‑3), also named Fetal liver kinase-2 (Flk-2) is a member of the class III receptor tyrosine kinase subfamily and plays a role in normal hematopoiesis. The classs III RTK family, which includes Flt-3, KIT, SCFR and CSF1R share a conserved architecture of an extracellular domain (ECD) with five immunoglobulin-like domains, a transmembrane domain and an intracellular region with a juxtamembrane domain and two intracellular tyrosine-kinase domains. The ECD of human Flt-3 shares 85% amino acid sequence identity with mouse Flt-3. Flt-3 expression has been detected in various tissues, including placenta, gonads, and tissues of nervous and hematopoietic origin (1). Among hematopoietic cells, the expression of Flt-3 was found to be restricted to the highly enriched stem/progenitor cell populations (2). The ligand for Flt-3 has been identified as Flt-3 ligand (Flt-3L or FL) and activation of Flt-3 signaling mediates cell survival, cell proliferation, and differentiation of hematopoietic progenitor cells (1). Flt-3 has been implicated in several diseases, including autoimmune diseases, such as rheumatoid arthritis, as well as in acute myeloid leukemia where around one-third of patients carry a Flt-3 mutation which drives the disease and is correlated with poor prognosis (3-5). Our Avi-tag Biotinylated Flt‑3 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

Long Name

fms-like Tyrosine Kinase 3

Alternate Names

CD135, Flk-2, Flt3, STK-1

Entrez Gene IDs

2322 (Human); 14255 (Mouse)

Gene Symbol

FLT3

UniProt

P36888.2

Additional Flt-3/Flk-2 Products

Product Documents for Recombinant Human Flt-3/Flk-2 Fc Chimera Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Manufacturing Specifications

  1. Kazi, J.U. and Rönnstrand, L. (2019) Physiol Rev. 99:1433.
  2. Matthews, W. et al. (1991) Cell 65:1143.
  3. Drexler, H.G. (1996) Leukemia 10:588.
  4. Grafone, T. et al. (2012) Oncol Rev. 6:e8.
  5. Stirewalt, D. and Radich, J. (2003) Nat. Rev. Cancer 3:650.

Product Specific Notices for Recombinant Human Flt-3/Flk-2 Fc Chimera Avi-tag Protein, CF

For research use only

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