Recombinant Human FSH alpha/beta Protein Best Seller

R&D Systems, part of Bio-Techne | Catalog # 5925-FS

R&D Systems, part of Bio-Techne
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Carrier Free
5925-FS-010/CF
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5925-FS-010
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Key Product Details

Source

CHO

Accession #

P01215 (FSH alpha) & P01225 (FSH beta)

Structure / Form

Noncovalently-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all FSH alpha/beta Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived FSH alpha/beta protein
Human FSH alpha 
Ala25-Ser116
Accession # P01215
Human FSH beta
Cys21-Glu129
Accession # P01225
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala25 (FSH alpha) & Cys21 (FSH beta)

Predicted Molecular Mass

10.2 kDa (FSH alpha) & 12.4 kDa (FSH beta)

SDS-PAGE

22-26 kDa, reducing conditions

Activity

Measured by its ability to induce cAMP accumulation in HEK293 human embryonic kidney cells transfected with human FSH R.
The ED50 for this effect is 25‑200 pg/mL.

Scientific Data Images for Recombinant Human FSH alpha/beta Protein

Recombinant Human FSH alpha/beta Protein Bioactivity

Recombinant Human FSH alpha/beta Protein Bioactivity

Recombinant Human FSH alpha/ beta (Catalog # 5925-FS) induces cAMP accumulation in the HEK293 human embryonic kidney cell line transfected with human FSH R. The ED50 for this effect is 25-200 pg/mL.
Recombinant Human FSH alpha/beta Protein SDS-PAGE

Recombinant Human FSH alpha/beta Protein SDS-PAGE

1 µg/lane of Recombinant Human FSH alpha/ beta was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing major bands at 20-25 kDa.
Recombinant Human FSH alpha/beta Protein Gel Filtration Chromatography

Recombinant Human FSH alpha/beta Protein Gel Filtration Chromatography

40 µg of Recombinant Human FSH alpha/ beta (blue line) was applied onto a Superdex 200 column. The peak eluted at approximately 44 kDa, form overlaid on the molecular weight markers (gray line), corresponds to the dimeric molecular mass.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 5925-FS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 5925-FS/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FSH alpha/beta

Follicle-Stimulating Hormone (FSH) is a 33 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit is also a component of Luteinizing Hormone (LH), Thyroid-Stimulating Hormone, and Chorionic Gonadotropin. The unique beta subunit confers the protein’s specific biological action and is responsible for the interaction with the FSH Receptor (1-3). The approximately 20 kDa alpha subunit of human FSH shares 73% and 72% aa sequence identity with the mouse and rat orthologs, respectively. The approximately 21 kDa human FSH beta subunit shares 85% and 82% aa sequence identity with the mouse and rat orthologs, respectively. FSH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Gonadotropin-Releasing Hormone from the hypothalamus; however, FSH secretion can also be stimulated by activins and inhibited by inhibins (4-6). FSH works in concert with LH to regulate female reproduction; FSH stimulates follicular growth and LH induces ovulation (7). FSH can also regulate estrogen formation by inducing the aromatase enzyme that converts androgen to estradiol (7, 8). In addition, it has been suggested to have a role in postmenopausal bone loss and in the neovascularization surrounding malignant tumors (9-11). In males, FSH is critical for sperm production (12). Mutations in the human FSH and FSHR genes are associated with reduced reproductive functioning in females (2, 11). Additionally, increased production of autoantibodies directed against FSH beta has been suggested to contribute to certain types of female infertility (2). Assisted reproductive technology commonly uses recombinant FSH to induce follicle growth (7).

References

  1. Fox, K.M. et al. (2001) Mol. Endocrinol. 15:378.
  2. Haller-Kikkatalo, K. et al. (2012) Clin. Dev. Immunol. 2012:762541.
  3. Jiang, X. et al. (2014) Mol. Cell. Endocrinol. 382:424.
  4. Findlay, J.K. (1993) Biol. Reprod. 48:15.
  5. Marshall, J.C. et al. (1993) Trans. Am. Clin. Climatol. Assoc. 104:31.
  6. Bernard, D.J. and S. Tran (2013) Biol. Reprod. 88:1.
  7. Raju, G.A. et al. (2013) J. Hum. Reprod. Sci. 6:227.
  8. Erickson, G.F. and A.J. Hsueh (1978) Endocrinology 102:1275.
  9. Sun, L. et al. (2006) Cell 125:247.
  10. Radu, A. et al. (2010) N. Engl. J. Med. 363:1621.
  11. Laan, M. et al. (2012) Curr. Opin. Endocrinol. Diabetes Obes. 19:220.
  12. Franchimont, P. et al. (1975) J. Reprod. Fertil. 44:335.

Long Name

Follicle Stimulating Hormone

Entrez Gene IDs

2488 (Human); 14308 (Mouse); 25447 (Rat)

Gene Symbol

FSHB

UniProt

P01215 (FSH alpha) & P01225 (FSH beta)

Additional FSH alpha/beta Products

Product Documents for Recombinant Human FSH alpha/beta Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 5925-FS (with carrier)
Download SDS 5925-FS/CF (carrier free)

Product Specific Notices for Recombinant Human FSH alpha/beta Protein

For research use only

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