Recombinant Human G-CSF Protein Best Seller
R&D Systems, part of Bio-Techne | Catalog # 214-CS
Key Product Details
Product Specifications
Source
E. coli-derived human G-CSF protein
Thr31-Pro204, with an N-terminal Met
Thr31-Pro204, with an N-terminal Met
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
18.8 kDa
SDS-PAGE
17-19 kDa, reducing conditions
Activity
Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. Shirafuji, N.et al. (1989) Exp. Hematol. 17:116.
The ED50 for this effect is 10-60 pg/mL.
The ED50 for this effect is 10-60 pg/mL.
Reviewed Applications
Read 2 reviews rated 5 using 214-CS in the following applications:
Scientific Data Images for Recombinant Human G-CSF Protein
Recombinant Human G-CSF Protein Bioactivity
Recombinant Human G-CSF (Catalog # 214-CS) stimulates cell proliferation of the NFS-60 mouse myelogenous leukemia lymphoblast cell line. The ED50 for this effect is 10-60 pg/mL.
Recombinant Human G-CSF Protein SDS-PAGE
1 μg/lane of Recombinant Human G-CSF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18.5 kDa.Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 214-CS
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetic Acid with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 214-CS/CF
| Formulation | Supplied as a 0.2 μm filtered solution in Acetic Acid. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Do not freeze.
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Background: G-CSF
In humans, two distinct cDNA clones for G-CSF, encoding 207 and 204 amino acid precursor proteins, have been isolated. Both proteins have a 30 amino acid signal peptide and have identical amino acid sequences except for a three amino acid insertion (deletion) at the 35th amino acid residue from the N-terminus of the mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.
In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.
Long Name
Granulocyte Colony Stimulating Factor
Alternate Names
C17orf33, CSF3, CSF3OS, Filgrastim, GCSF, Lenograstim, Pluripoietin
Gene Symbol
CSF3
UniProt
Additional G-CSF Products
Product Documents for Recombinant Human G-CSF Protein
Product Specific Notices for Recombinant Human G-CSF Protein
For research use only
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