Recombinant Human GIPR Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11088-GI

R&D Systems, part of Bio-Techne
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11088-GI-050
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Key Product Details

Source

HEK293

Accession #

P48546.1

Conjugate

Unconjugated

Applications

Bioactivity

View all GIPR Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human GIPR protein
Human GIPR
(Gly26-Gln138)
Accession # P48546.1		 IEGRMD Human IgG1 Fc
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly26

Predicted Molecular Mass

40 kDa

SDS-PAGE

45-59 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Biotinylated Human GIP Peptide is captured on a Streptavidin Coated Plate  (Catalog # CP004), it binds to Recombinant Human GIPR Fc Chimera. The ED50 for this binding is 20.0-100 ng/mL.

Scientific Data Images for Recombinant Human GIPR Fc Chimera Protein, CF

Recombinant Human GIPR Fc Chimera Protein Binding Activity.

When Biotinylated Human GIP Peptide is captured on a Streptavidin Coated Plate (CP004), it binds to Recombinant Human GIPR Fc Chimera Protein (Catalog # 11088-GI). The ED50 for this binding is 20.0-100 ng/mL.

Recombinant Human GIPR Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human GIPR Fc Chimera Protein (Catalog # 11088-GI) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-59 kDa and 90-120 kDa, respectively.

Formulation, Preparation and Storage

11088-GI
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GIPR

Human Gastric Inhibitory Polypeptide Receptor (GIPR) is a transmembrane G protein coupled receptor that is mainly found in beta-cells within the pancreas (1). GIPR has 117 aa extracellular domain on its N-terminus, a central region consisting of seven transmembrane domains and a 68 aa C-terminal cytoplasmic domain that is responsible for intracellular transduction with the G-Protein (2). GIPR has three known isoforms produced by alternative splicing. The extracellular domain of human GIPR shows 76.3% and 81.2% amino acid identity with mouse and rat homolog, respectively. When originally discovered, GIPR was thought to have a role of inhibiting the secretion of gastrin and gastric acid (1). However, it was subsequently discovered that GIPR's main function was to stimulate the release of insulin in the presence of elevated blood glucose levels (1). GIPR has been found to bind to glucagon-like peptide-1 (GIP) and cascades downstream to release insulin (1). GIP and its receptor (GIPR) are of high pharmacological interest, since expression of GIPR are found in different organs and systems, especially in identification and design of new molecules for the treatment of diabetes mellitus and obesity (3-6). GIPR has also been shown to have an indirect relation with bone health and density. Mouse overexpressing GIP and GIPR had increased levels of osteoblasts and an overall decrease in age-related bone loss, while mice with GIPR knockout showed a decrease in overall bone mass and a higher level of compromised bone mass (7). Targeted Radionuclide Therapy (TRT) against GIPR positive cancer cells showed a significant increase of cell cycle arrest, specifically at the G2 and M phase, along with extensive DNA damage (8).

References

  1. YAMADA, Y. et al. (1995). Genomics 29:773.
  2. Parthier, C. et al. (2007). Proc.Natl. Acad. Sci. U.S.A. 104:13942.
  3. Sherman, S.K. et at. Surgery. (2013) 154(6): . doi:10.1016/j.surg.2013.04.052.
  4. Yagub, T. et al. (2010) Molecular Pharmacology. 77(4):547.
  5. Volz, A. et al. (1995) Febs Letters. 373(1):23.
  6. Kieffer, T.J. et al. (2003) Trends Pharmacol Sci 24:110.
  7. Torekov, S.S. et al. (2014) J Clin Endocrinol Metab. 99:E729.
  8. Shi, X, et al. (2021) Bioconjug Chem. 32:1763.

Long Name

Gastric Inhibitory Polypeptide Receptor

Alternate Names

PGQTL2

Entrez Gene IDs

2696 (Human); 381853 (Mouse); 25024 (Rat)

Gene Symbol

GIPR

UniProt

P48546.1

Additional GIPR Products

Product Documents for Recombinant Human GIPR Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human GIPR Fc Chimera Protein, CF

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