Recombinant Human Glucokinase/GCK Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7840-GK

R&D Systems, part of Bio-Techne
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7840-GK-020
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Key Product Details

Source

E. coli

Accession #

P35557

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Glucokinase/GCK Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Glucokinase/GCK protein
Met 6-His tag Human Cystatin A
(Met1-Phe98)
Accession # P01040		 GS Human Glucokinase/GCK
(Val16-Gln465)
Accession # P35557
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

63 kDa

SDS-PAGE

60-65 kDa, reducing conditions

Activity

Measured by its ability to transfer phosphate from ATP to glucose.
The specific activity is >265 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

7840-GK
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, Brij-35 and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Glucokinase/GCK

Hexokinases phosphorylate hexose to form hexose 6‑phosphate, the first step for hexose metabolism. There are four mammalian hexokinases (I, II, III and IV) and hexokinase IV is commonly known as glucose kinase (GCK). Unlike hexokinase I, II and III, which have high affinity for glucose and are strongly inhibited by the product, glucose‑6‑phosphate (1), GCK has much lower affinity for glucose and is not inhibited by the product (2, 3). Consequently, GCK has a Km for glucose of approximately 7 mM (4), which is 100 times higher than that of hexokinase I, II, and III. This unique enzymatic property of GCK allows it to respond to blood glucose levels and contribute to the maintenance of blood glucose levels within the normal physiological range of 4 mM to 6 mM. In the pancreatic islets, GCK serves as a glucose sensor to control insulin release in the beta cells, and to control glucagon release in the alpha cells (5). In hepatocytes, GCK responds to changes of ambient glucose levels by increasing or reducing glycogen synthesis. Mutations in GCK have been associated with non‑insulin‑dependent diabetes mellitus (6, 7), maturity‑onset diabetes of the young type 2 (8), and hyperinsulinemia of infancy (9). The enzyme activity was measured using a phosphatase coupled kinase assay (4).

References

  1. Aleshin, A.E. et al.(1998) Structure 6:39.
  2. Takeda, J. et al. (1993) J. Biol. Chem. 268:15200.
  3. Lange, A.J. et al. (1991) Biochem. J. 277:159.
  4. Wu, Z.L. (2011) PLoS ONE 6(8):e23172.
  5. Xu, L.Z. et al. (1995) J. Biol. Chem. 270:9939.
  6. Gidh-Jain, M. et al. (1993) Proc. Natl. Acad. Sci. U. S. A. 90:1932.
  7. Stoffel, M. et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89:7698.
  8. Hattersley, A.T. et al. (1992) Lancet 339:1307.
  9. Gloyn, A.L. et al. (2003) Diabetes 52:2433.

Long Name

Glucose Kinase

Alternate Names

FGQTL3, GCK, GLK, Hexokinase-4, Hexokinase-D, HHF3, HK4, HXKP, LGLK

Entrez Gene IDs

2645 (Human); 103988 (Mouse); 24385 (Rat)

Gene Symbol

GCK

UniProt

P35557 (Human)

Additional Glucokinase/GCK Products

Product Documents for Recombinant Human Glucokinase/GCK Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Glucokinase/GCK Protein, CF

For research use only

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