Recombinant Human Glutathione Reductase Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8866-GR

R&D Systems, part of Bio-Techne
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8866-GR-100
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Key Product Details

Source

E. coli

Accession #

P00390

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Glutathione Reductase Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Glutathione Reductase protein
Met44-Arg522, with an N-terminal Met and 6-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

53 kDa

SDS-PAGE

53 kDa, reducing conditions

Activity

Measured by its ability to reduce oxidized glutathione to glutathione in an NADPH-dependent manner.
The specific activity is >15,000 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8866-GR
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Glutathione Reductase

Glutathione Reductase (GR) is a homodimeric flavoprotein disulfide oxidoreductase that plays a critical role in the prevention of oxidative damage within the cell (1, 2). Glutathione Reductase helps to maintain appropriate levels of intracellular reduced glutathione (GSH) which is essential for maintaining the normal structure of red blood cells and for keeping hemoglobin in the ferrous state (3). Glutathione Reductase together with its co-factor, NADPH, catalyzes the reduction of oxidized glutathione (glutathione disulfide, GSSG) to glutathione. GSH is also a reactant for Glutathione Peroxidase, which converts hydrogen peroxide hydrogen peroxide (H2O2) into water (4). Glutathione Reductase is expressed as both mitochondrial and cytosolic isoforms, as determined by the presence or absence of an N-terminal transit peptide. Human Glutathione Reductase shares 86% and 76% amino acid sequence identity with mouse and rat Glutathione Reductase, respectively (5, 6). Alternative splicing of human Glutathione Reductase generates additional isoforms with deletions in the central pyridine nucleotide-disulfide oxidoreductase domain (7).

References

  1. Deponte, M. (2013) Biochim. Biophys. Acta 1830:3217.
  2. Fujii, J. et al. (2011) J. Clin. Biochem. Nutr. 49:70.
  3. Tsantes, A.E. et al. (2006) Antioxid. Redox Signal. 8:1205.
  4. Lubos, E. et al. (2011) Antioxid. Redox Signal. 15:1957.
  5. Tutic, M. et al. (1990) Eur. J. Biochem. 188:523.
  6. Krauth-Siegel, R.L. et al. (1982) Eur. J. Biochem. 121:259.
  7. Satoh, N. et al. (2010) Biochem. Genet. 48:816.

Alternate Names

GLUR, GRase, GRD1, GSR, HEL-752

Entrez Gene IDs

2936 (Human); 14782 (Mouse); 116686 (Rat)

Gene Symbol

GSR

UniProt

P00390

Additional Glutathione Reductase Products

Product Documents for Recombinant Human Glutathione Reductase Protein, CF

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Product Specific Notices for Recombinant Human Glutathione Reductase Protein, CF

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