Recombinant Human Glypican 4 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9195-GP

R&D Systems, part of Bio-Techne
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9195-GP-050
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Key Product Details

Source

CHO

Accession #

O75487

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Glypican 4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Glypican 4 protein
Human Glypican 4
(Ala23-Ser529)
Accession # O75487		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala23 & Ser355

Predicted Molecular Mass

84 kDa

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Glypican 4 Fc Chimera is coated at 500 ng/mL (100 μL/well), the concentration of recombinant human FGF basic that produces 50% of the optimal binding response is 0.75-4.5 ng/mL.

Scientific Data Images for Recombinant Human Glypican 4 Fc Chimera Protein, CF

Recombinant Human Glypican 4 Fc Chimera Protein Bioactivity

Recombinant Human Glypican 4 Fc Chimera Protein Bioactivity

When Recombinant Human Glypican 4 Fc Chimera (Catalog # 9195-GP) is coated at 500 ng/mL 100 µL/well), the concentration of Recombinant Human FGF basic 146 aa (Catalog # 233-FB) that produces 50% of the optimal binding response is 0.75-4.5 ng/mL.

Formulation, Preparation and Storage

9195-GP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 4

Glypican 4, also known as K-Glypican, is an approximately 220 kDa GPI-anchored heparan sulfate proteoglycan with a 60 kDa protein core (1, 2). It is expressed in brain, kidney, adrenal gland, and fat tissue (1, 3) and binds to basic FGF (2). Mature human Glypican 4 shares 97% and 96% amino acid (aa) sequence identity with mouse and rat Glypican 4, respectively. An alternative splice isoform lacks the N-terminal 70 aa including the signal peptide. In the developing brain, Glypican 4 is found in lateral ventricles surrounding the telencephalon, the dentate gyrus, proliferating neuroepithelial cells, and neural precursors (1, 2). It inhibits the dopaminergic differentiation of neurons (4). A 30 kDa cleaved form of Glypican 4 binds in cis to PTP sigma and contributes to excitatory synapse development and function (5). Glypican 4 is differentially expressed between adipose tissue depots (3). A soluble form can be released by adipocytes and circulates at elevated levels in obese patients with insulin resistance (3, 6). This form binds and enhances signaling through the Insulin R, and it also supports adipocyte differentiation (3). In zebrafish, Glypican 4 is required for cartilage formation and cardiomyocyte differentiation (7, 8).

References

  1. Watanabe, K. et al. (1995) J. Cell Biol. 130:1207.
  2. Hagihara, K. et al. (2000) Dev. Dyn. 219:353.
  3. Ussar, S. et al. (2012) Diabetes 61:2289.
  4. Fico, A. et al. (2014) J. Neurosci. 34:8318.
  5. Ko, J.S. et al. (2015) Proc. Natl. Acad. Sci. USA 112:1874.
  6. Zhu, H.J. et al. (2014) J. Endocrinol. Metab. 99:E2697.
  7. Sisson, B.E. et al. (2015) Mech. Dev. 138:279.
  8. Strate, I. et al. (2015) Development 142:1767.

Alternate Names

GPC4, K-glypican

Entrez Gene IDs

2239 (Human); 14735 (Mouse); 317322 (Rat)

Gene Symbol

GPC4

UniProt

O75487

Additional Glypican 4 Products

Product Documents for Recombinant Human Glypican 4 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Glypican 4 Fc Chimera Protein, CF

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