Recombinant Human gp130 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 671-GP
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human gp130 protein
| Human gp130 (Glu23-Ile618) Accession # P40189 |
DIEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu23
Predicted Molecular Mass
95 kDa (monomer)
SDS-PAGE
115-130 kDa, reducing conditions
Activity
Measured by its ability to inhibit IL-6-dependent proliferation of the 7TD1 mouse hybridoma cells in the presence of Recombinant Human IL-6 R alpha (Catalog # 227-SR).
The ED50 for this effect is 0.65-6.5 ng/mL.
The ED50 for this effect is 0.65-6.5 ng/mL.
Reviewed Applications
Read 1 review rated 5 using 671-GP in the following applications:
Formulation, Preparation and Storage
671-GP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: gp130
Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.
Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage or the membrane-bound receptor of from alternative splicing, has been detected in human serum. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.
References
- Narazaki, M. et al. (1993) Blood 82:1120.
- Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
Long Name
Glycoprotein 130
Alternate Names
CD130, IL6ST
Gene Symbol
IL6ST
UniProt
Additional gp130 Products
Product Documents for Recombinant Human gp130 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human gp130 Fc Chimera Protein, CF
For research use only
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