Recombinant Human GPR111 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6494-GP

R&D Systems, part of Bio-Techne
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6494-GP-050
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Key Product Details

Source

CHO

Accession #

Q8IZF7.2

Conjugate

Unconjugated

Applications

Bioactivity

View all GPR111 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human GPR111 protein
Cys19-Ser375, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys19

Predicted Molecular Mass

40.9 kDa

SDS-PAGE

55-66 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of U-87 MG human glioblastoma/astrocytoma cells.
The ED50 for this effect is 1-5 μg/mL.

Formulation, Preparation and Storage

6494-GP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GPR111

GPR111, also known as PGR20, is an approximately 70 kDa member of the LN-7TM family of adhesion G protein-coupled receptors. Like other LN-7TM family proteins, it contains an extended N-terminal extracellular region followed by a series of seven transmembrane (TM) segments and a short C-terminal cytoplasmic tail (1, 2). The N-terminal domain contains a mucin-like stalk and a juxtamembrane region that resembles the GPS motif found in other LN-7TM proteins. The GPS motif is a component of the conserved GAIN domain which mediates the autoproteolysis and shedding of a wide range of proteins including LN-7TM proteins (3). The GPS-like motif in GPR111, however, is divergent from the consensus motif and does not appear to be cleavable (3, 4). Alternative splicing of human GPR111 generates a long isoform with a 92 aa substitution for the N-terminal 24 residues as well as a 19 aa substitution in the final TM segment and cytoplasmic tail. This recombinant protein product corresponds to the N-terminal extracellular domain of the shorter isoform (aa 19-375). Within this region, human GPR111 shares approximately 68% aa sequence identity with mouse and rat GPR111. GPR111 is expressed in squamous epithelia of the skin, esophagus, tongue epidermis, and stomach (4).

References

  1. Fredriksson, R. et al. (2002) FEBS Lett. 531:407.
  2. Bjarnadottir, T.K. et al. (2004) Genomics 84:23.
  3. Arac, D. et al. (2012) EMBO J. 31:1364.
  4. Promel, S. et al. (2012) Dev. Dyn. 241:1591.

Long Name

G-Protein-Coupled Receptor 111

Alternate Names

PGR20

Entrez Gene IDs

222611 (Human); 435529 (Mouse); 301269 (Rat)

Gene Symbol

ADGRF2

UniProt

Q8IZF7.2

Additional GPR111 Products

Product Documents for Recombinant Human GPR111 Protein, CF

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Product Specific Notices for Recombinant Human GPR111 Protein, CF

For research use only

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