Recombinant Human Granzyme A Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2905-SE
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Granzyme A protein
Cys26-Val262, with a C-terminal 10-His tag
Cys26-Val262, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Cys26
Predicted Molecular Mass
28 kDa
SDS-PAGE
Multiple bands between 29-33 kDa, reducing conditions
Activity
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >5,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2905-SE
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES, NaCl and CaCl2 with Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM HEPES, 150 mM NaCl and 10 mM CaCl2, pH 7.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Granzyme A
Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is the most abundant protease in CTL and NK cells. It induces caspase‑independent cell death when introduced into target cells by perforin (1). Human granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 1‑26), a propeptide (residues 27-28) and a mature chain (residues 29-262 ) (2). The purified recombinant human Granzyme A consists of residues 26 to 262. After being activated by lysyl endopeptidase, it cleaves a thioester substrate as described in Activity Assay Protocol.
References
- Lieberman, J. and Z. Fan (2003) Curr. Opin. Immunol. 15:553.
- Gershenfeld, H.K. et al. (1988) Proc. Natl. Acad. Sci. USA 85:1184.
Alternate Names
CTL Tryptase, CTLA3, Fragmentin-1, GZMA, HF, HFSP
Gene Symbol
GZMA
UniProt
Additional Granzyme A Products
Product Documents for Recombinant Human Granzyme A Protein, CF
Product Specific Notices for Recombinant Human Granzyme A Protein, CF
For research use only
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