Recombinant Human Guanylyl Cyclase C Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10575-GC

R&D Systems, part of Bio-Techne
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10575-GC-050
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Key Product Details

Source

HEK293

Accession #

P25092.2

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Guanylyl Cyclase C/GUCY2C Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Guanylyl Cyclase C/GUCY2C protein
Human Guanylyl Cyclase C/GUCY2C
(Ser21-Gln430)
Accession # P25092.2		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser21

Predicted Molecular Mass

73 kDa

SDS-PAGE

100-115 kDa, under reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Human Guanylyl Cyclase C Fc Chimera Protein, CF

Recombinant Human Guanylyl Cyclase C Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Human Guanylyl Cyclase C Fc Chimera Protein (Catalog # 10575-GC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 100-115 kDa and 200-230 kDa, respectively.

Formulation, Preparation and Storage

10575-GC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Guanylyl Cyclase C/GUCY2C

Guanylyl Cyclase C (GUCY2C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (GC) family expressed by intestinal epithelial cells from the duodenum to rectum. It was first identified as the intestinal epithelial receptor regulating fluid and electrolyte transport in the secretory diarrhea induced by bacterial enterotoxins (1). There are 7 known members of the GC family (GC-A through GC-G) and they catalyze the conversion of guanosine triphosphate to cyclic guanosine monophosphate (cGMP) and pyrophosphate (2-4). Mature human GUCY2C consists of an extracellular domain (ECD) with a ligand binding domain, a transmembrane segment and a cytoplasmic region possessing a kinase and GC catalytic domain. The ECD of human GUCY2C shares 70% and 72% amino acid sequence identity with the ECD of mouse and rat GUCY2C, respectively. Endogenous ligands of GUCY2C include guanylin and uroguanylin (5). GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis. GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior (5-7).

References

  1. Lucas K. et al. (2000) Pharmacol. Rev. 52:375.
  2. Potter, L.R. (2011) Cell Signal. 23:1921.
  3. Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
  4. Gibbons, A.V. et al. (2013) Cancer Res. 73:22.
  5. Erik, S. et al. (2016) Mol. Pharmacol. 90:199.
  6. Gibbons, A.V. et al. (2013) Cancer Res. 73:22.
  7. Gong, R. et al. (2011) Science 333:1642.

Alternate Names

DIAR6, GC-C, GCC, Guanylate Cyclase C, GUC2C, GUCY2C, MUCIL, STA Receptor, StAR

Entrez Gene IDs

2984 (Human); 14917 (Mouse); 25711 (Rat)

Gene Symbol

GUCY2C

UniProt

P25092.2

Additional Guanylyl Cyclase C/GUCY2C Products

Product Documents for Recombinant Human Guanylyl Cyclase C Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Guanylyl Cyclase C Fc Chimera Protein, CF

For research use only

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