Recombinant Human Guanylyl Cyclase C His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2157-GC

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
2157-GC-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

HEK293

Accession #

P25092.2

Conjugate

Unconjugated

Applications

Bioactivity

View all Guanylyl Cyclase C/GUCY2C Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human Guanylyl Cyclase C/GUCY2C protein
Ser21-Gln430, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser21

Predicted Molecular Mass

47 kDa

SDS-PAGE

73-81 kDa, reducing conditions

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant Human Guanylyl Cyclase C His-tag Protein, CF

Recombinant Human Guanylyl Cyclase C His-tag Protein SDS-PAGE

Recombinant Human Guanylyl Cyclase C His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human Guanylyl Cyclase C/GUCY2C was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 73-81 kDa.

Formulation, Preparation and Storage

2157-GC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Guanylyl Cyclase C/GUCY2C

GUCY2C (Guanylyl Cyclase C), also known as heat-stable enterotoxin receptor, is a type I transmembrane protein of the guanylate cyclase (gc) family that signal by producing cGMP (1, 2). GUCY2C contains a 23 amino acid (aa) signal sequence, a 407 aa extracellular region (ECD), a 24 aa transmembrane (TM) segment and a 619 aa cytoplasmic domain (3). The ECD of human GUCY2C shares 71% and 72% aa identity with mouse and rat GUCY2C, respectively (4). GUCY2C was first identified as the intestinal epithelial receptor regulating fluid and electrolyte transport in the secretory diarrhea induced by bacterial enterotoxins (5). Endogenous ligands of GUCY2C include guanylin and uroguanylin (6). GUCY2C in epithelial cells plays an important role in cell dynamics and homeostatic balance of proliferation, metabolism, and differentiation that organizes the guanylyl cyclase C hormone axis (2, 6). GUCY2C is also expressed in the brain and is implicated in attention deficiency and hyperactive behavior (2, 7). CAR-T cell therapy utilizing GUCY2C to treat metastatic colorectal cancer is currently being explored (8).

References

  1. Arshad, N. et al. (2013) J. Biol. Chem. 288:3907.
  2. Gibbons, A. V. et al. (2013) Cancer Res. 73:22.
  3. de Sauvage, F. J. et al. (1991) J. Biol. Chem. 266:17912.
  4. Singh S. et al. (1991) Biochem. Biophys. Res. Comm. 179:1455.
  5. Lucas K. et al. (2000) Pharmacol Rev. 52:375.
  6. Erik, S. et al. (2016) Mol. Pharmacol. 90:199.
  7. Gong, R. et al. (2011) Science. 333:1642.
  8. Magee, MS. et al. (2018) Cancer Immuno Res. 6:509.

Alternate Names

DIAR6, GC-C, GCC, Guanylate Cyclase C, GUC2C, GUCY2C, MUCIL, STA Receptor, StAR

Entrez Gene IDs

2984 (Human); 14917 (Mouse); 25711 (Rat)

Gene Symbol

GUCY2C

UniProt

P25092.2

Additional Guanylyl Cyclase C/GUCY2C Products

Product Documents for Recombinant Human Guanylyl Cyclase C His-tag Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Guanylyl Cyclase C His-tag Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.