Recombinant Human HABP1/C1QBP Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 5155-HB
Key Product Details
Product Specifications
Source
E. coli-derived human HABP1/C1QBP protein
His75-Gln282, with an N-term 2-Met tag and a C-terminal 6-His tag
His75-Gln282, with an N-term 2-Met tag and a C-terminal 6-His tag
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
24.7 kDa
SDS-PAGE
34 kDa, reducing conditions
Activity
Measured by its ability to bind human C1q in a functional ELISA.
Formulation, Preparation and Storage
5155-HB
| Formulation | Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile deionized water.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: HABP1/C1QBP
Hyaluronan binding protein 1 (HABP1), also known as C1qBP/C1qR and p32, is an ubiquitous acidic glycoprotein that functions both in spermatogenesis and as a receptor for proinflammatory molecules (1, 2). HABP1 is synthesized with a 73 amino acid (aa) N-terminal propeptide (3). The 32 kDa mature human HABP1, which contains a MAM33-like sequence, shares 90% aa sequence identity with mouse and rat HABP1. HABP1 assembles into a doughnut shaped trimer with the negatively charged residues asymmetrically distributed on one face and lining the channel of the complex (4). HABP1 can be cleaved by cell surface MMP-14/MT1-MMP, a protease important in angiogenesis and tumor metastasis (5). Cell surface HABP1 binds a wide range of extracellular molecules including hyaluronan, vitronectin, complement component C1q, HMW kininogen, and bacterial and viral proteins (2, 3, 6 - 8). Within the cell, HABP1 binds to molecules containing the C1q globular domain, multiple isoforms of PKC, mitochondrial Hrk, the cytoplasmic tails of adrenergic and GABA-A receptors, the mRNA splicing factor ASF/SF2, and the CBF transcription factor (9 - 14). Apoptosis and direct phosphorylation by Erk1/2 induces HABP1 translocation to the nucleus (15).
References
- Thakur, S.C. et al. (2006) J. Androl. 27:604.
- Peerschke, E.I.B. and B. Ghebrehiwet (2007) Immunobiology 212:333.
- Grebrehiwet, B. et al. (1994) J. Exp. Med. 179:1809.
- Jiang, J. et al. (1999) Proc. Natl. Acad. Sci. 96:3572.
- Rozanov, D.V. et al. (2002) J. Biol. Chem. 277:9318.
- Deb, T.B. and K. Datta (1996) J. Biol. Chem. 271:2206.
- Lim, B.L. et al. (1996) J. Biol. Chem. 271:26739.
- Waggoner, S.N. et al. (2007) J. Leukoc. Biol. 82 epub.
- Innamorati, G. et al. (2006) Cell. Signal. 18:761.
- Robles-Flores, M. et al. (2002) J. Biol. Chem. 277:5247.
- Sunayama, J. et al. (1994) Cell Death Differ. 11:771.
- Schaerer, M.T. et al. (2001) J. Biol. Chem. 276:26597.
- Peterson-Mahrt, S.K. et al. (1999) EMBO J. 18:1014.
- Chattopadhyay, C. et al. (2004) Nucleic Acids Res. 32:3632.
- Majumdar, M. et al. (2002) Biochem. Biophys. Res. Commun. 291:829.
Long Name
Hyaluronic Acid-binding Protein
Alternate Names
C1QBP, gC1qBP, gC1qR, SF2p32
Gene Symbol
C1QBP
UniProt
Additional HABP1/C1QBP Products
Product Documents for Recombinant Human HABP1/C1QBP Protein, CF
Product Specific Notices for Recombinant Human HABP1/C1QBP Protein, CF
For research use only
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