Recombinant Human Hepassocin/FGL1 Avi Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10573

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

Q08830.3

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all Hepassocin/FGL1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Hepassocin/FGL1 protein
Avi-tag MD Human IgG1 Fc
(Pro100-Lys330)		 IEGR Human FGL1
(Leu23-Ile312)
Accession # Q08830.3
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly of Avi-tag

Predicted Molecular Mass

63 kDa

SDS-PAGE

59-68 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human LAG-3 Fc Chimera Protein  (Catalog # 2319-L3) is immobilized at 2.50 µg/mL (100 µL/ well), Biotinylated Recombinant Human Hepassocin/FGL1 Avi-tag Fc Chimera (Catalog # AVI10573) binds with an ED50 of 0.250-2.50 μg/mL.

Scientific Data Images for Recombinant Human Hepassocin/FGL1 Avi Fc Chimera Protein, CF

Biotinylated Recombinant Human Hepassocin/FGL1 Avi-tag Fc Chimera Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Recombinant Human LAG-3 Fc Chimera Protein (2319-L3) is immobilized at 2.50 µg/mL (100 µL/ well), Biotinylated Recombinant Human Hepassocin/FGL1 Avi-tag Fc Chimera (Catalog # AVI10573) binds with an ED50 of 0.250-2.50 μg/mL.

Biotinylated Recombinant Human Hepassocin/FGL1 Avi-tag Fc Chimera Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human Hepassocin/FGL1 Avi-tag Fc Chimera Protein (Catalog # AVI10573) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 59-68 kDa and 120-140 kDa, respectively.

Formulation, Preparation and Storage

AVI10573
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepassocin/FGL1

Hepassocin, also known as Hepatocyte-derived fibrinogen-related protein 1 (HFREP-1) and Fibrinogen-Like Protein 1 (FGL1) (1), is a liver-specific secreted protein belonging to the fibrinogen superfamily whose members share a fibrinogen domain at their C-termini (2). Human Hepassocin/FGL1 is a secreted homodimer consisting of 312 amino acids (aa) with a 22 aa signal sequence and a 290 aa mature protein (3). Mouse and rat Hepassocin/FGL1 share approximately 84% and 83% amino acid identity with human Hepassocin/FGL1, respectively. Human Hepassocin/FGL1 binds LAG-3 through its fibrinogen-like domain independently of MHC class II (4). Hepassocin/FGL1 inhibits antigen-specific T cell activation, with its elevated presence in plasma of cancer patients indicating poor prognoses (4). Other than this role in cancer progression, Hepassocin/FGL1 also has restorative function for liver cells. It is upregulated during liver regeneration following partial hepatectomy (5), and stimulates proliferation of hepatocytes in vivo and improves prognoses with fulminant hepatic failure in rats (6). Its expression is regulated in Hep G2 cells by interleukin-6 (IL-6) and is found in the serum in both bound and unbound states as an acute phase reactant (7). Our Avi-tag Biotinylated human Hepassocin/FGL1 Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Yamamoto, T. et al. (1993) Biochem. Biophys. Res. Commun. 2:681.
  2. Zhang, S.M. et al. (2008) Innate Immun. 14:175.
  3. Hara, H. et al. (2001) Biochim. Biophys. Acta. 1520:45.
  4. Wang, J. et al. (2019) Cell. 176:334.
  5. Yu, HT. et al. (2009) J. Biol. Chem. 284:13335.
  6. Li, C.Y. et al. (2010) Gut. 59:817.
  7. Liu, Z. and C. Ukomadu. (2008) Biochem. Briophys. Res. Commun. 365:729.

Long Name

Fibrinogen Like 1

Alternate Names

FGL1, Fibrinogen-like-protein 1, FREP1

Entrez Gene IDs

2267 (Human); 234199 (Mouse); 246186 (Rat); 102118020 (Cynomolgus Monkey)

Gene Symbol

FGL1

UniProt

Q08830.3

Additional Hepassocin/FGL1 Products

Product Documents for Recombinant Human Hepassocin/FGL1 Avi Fc Chimera Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human Hepassocin/FGL1 Avi Fc Chimera Protein, CF

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