Recombinant Human HGF (NS0-expressed) GMP Protein, CF GMP
R&D Systems, part of Bio-Techne | Catalog # 294-GMP
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked heterodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human HGF protein
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
Manufactured and tested under cGMP guidelines.
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
Manufactured and tested under cGMP guidelines.
Purity
>95%, by SDS-PAGE with silver staining, under reducing conditions.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Amino acid sequencing of the alpha chain was blocked, suggesting it is consistent with Gln32
Gln32-Arg-Lys-Arg-Arg-Asn-Thr-Ile-His-Glu ( alpha-chain)
Val495-Val-Asn-Gly-Ile-Pro-Thr-Arg-Thr-Asn ( beta-chain)
Gln32-Arg-Lys-Arg-Arg-Asn-Thr-Ile-His-Glu ( alpha-chain)
Val495-Val-Asn-Gly-Ile-Pro-Thr-Arg-Thr-Asn ( beta-chain)
Predicted Molecular Mass
alpha chain: 53.7 kDa
beta chain: 26 kDa
beta chain: 26 kDa
SDS-PAGE
71-85 kDa, non-reducing conditions
56-65 kDa, and 30-40 kDa, reducing conditions
56-65 kDa, and 30-40 kDa, reducing conditions
Activity
Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883.
The ED50 for this effect is equal or less than 4.00 ng/mL.
The specific activity of recombinant human HGF is >1.0 x 106 IU/mg, which is calibrated against the human HGF WHO International Standard (NIBSC code: 96/564).
The ED50 for this effect is equal or less than 4.00 ng/mL.
The specific activity of recombinant human HGF is >1.0 x 106 IU/mg, which is calibrated against the human HGF WHO International Standard (NIBSC code: 96/564).
Host Cell Protein
<5 ng per µg of protein when tested by ELISA.
Mycoplasma
Negative for Mycoplasma.
Scientific Data Images for Recombinant Human HGF (NS0-expressed) GMP Protein, CF
Recombinant Human HGF (NS0-expressed) GMP Protein Bioactivity
Recombinant Human HGF GMP induces IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is equal or less than 4.00 ng/mL.
Recombinant Human HGF (NS0-expressed) GMP Protein SDS-PAGE
1 μg/lane of GMP-grade Recombinant Human HGF (Catalog # 294-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing R bands at 30-40 and 56-65 kDa and NR bands at 71-85 kDa, respectively.Formulation, Preparation and Storage
294-GMP
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in PBS. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: HGF
HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE‑like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive
disulfide‑linked HGF with a 60 kDa alpha and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase‑like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the up‑regulation of integrin alpha2 beta1 in epithelial cells by a selective increase in alpha2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin‑1 alpha ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH‑stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).
References
- Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
- Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
- Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
- Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
- Nakamura, T. et al. (1989) Nature 342:440.
- Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
- Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
- Corso, S. et al. (2005) Trends Mol. Med. 11:284.
- Maeshima, A. et al. (2000) Kid. Int. 58:1511.
- Montesano, R. et al. (1991) Cell 67:901.
- Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
- Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
- Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
- Mineo, R. et al. (1994) Endocrinology 145:4355.
- Urbanek, K. et al. (2005) Circ. Res. 97:663.
Long Name
Hepatocyte Growth Factor
Alternate Names
DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF
Entrez Gene IDs
Gene Symbol
HGF
UniProt
Additional HGF Products
Product Documents for Recombinant Human HGF (NS0-expressed) GMP Protein, CF
Manufacturing Specifications
GMP ProteinsR&D Systems, a Bio-Techne Brand's GMP proteins are produced according to relevant sections of the following documents: USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and Eu. Ph. 5.2.12, Raw Materials of Biological Origin for the Production of Cell-based and Gene Therapy Medicinal Products.
R&D Systems' quality focus includes:
- Manufactured and tested under an ISO 9001:2015 and ISO 13485:2016 certified quality system
- Documented processes and QA control of documentation and process changes
- Personnel training programs
- Raw material testing and vendor qualification/monitoring
- Fully validated equipment, processes and test methods
- Equipment calibration schedules using a computerized calibration program
- Facility maintenance, safety programs and pest control
- Material review process for variances
- Monitoring of stability over product shelf-life
R&D Systems strives to provide our customers with the analytical characteristics of each product so that customers may determine whether our products are appropriate for their research. The Certificate of Analysis provided contains the following lot specific information:
- N-terminal amino acid analysis, SDS-PAGE analysis, and endotoxin level (as determined by LAL assay) performed on each bulk QC lot, not on individual bottlings of each QC lot
- Post-bottling lot-specific bioassay results (compliance with an established range) and results of microbial testing according to USP
- Host Cell Protein testing performed by ELISA
- Mycoplasma testing by ribosomal RNA hybridization assay
Additional testing and documentation requested by the customer can be arranged at an additional cost.
Production records and facilities are available for examination by appropriate personnel on-site at R&D Systems in Minneapolis, Minnesota USA.
R&D Systems sells GMP grade products for preclinical or clinical ex vivo use. They are not for in vivo use. Please read the following End User Terms prior to using this product.
Product Specific Notices for Recombinant Human HGF (NS0-expressed) GMP Protein, CF
END USER TERMS OF USE OF PRODUCT
The following terms are offered to you upon your acceptance of these End User Terms of Use of Product. By using this product, you indicate your acknowledgment and agreement to these End User Terms of Use of Product. If you do not agree to be bound by and comply with all of the provisions of these End User Terms of Use of Product, you should contact your supplier of the product and make arrangements to return the product.
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The End User is aware that R&D Systems, Inc. sells GMP products for preclinical or clinical ex vivo use and not for in vivo use. The End User further agrees, as a condition of the sale of R&D Systems' GMP products that: a) the End User will not use this GMP Product in any procedure wherein the product may be directly or indirectly administered to humans, unless the End User has obtained, or prior to their use will have obtained, an Investigational New Drug (IND) exemption from the FDA and will use the product only in accordance with the protocols of such IND and of the Institutional Review Board overseeing the proposed research, or b) the End User will use the products outside of the United States in accordance with the protocols of research approved by the Institutional Review Board or authorized ethics committee and regulatory agencies to which the End User is subject to in their territory.
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For preclinical, or clinical ex vivo use
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