Recombinant Human His6-E6AP/UBE3A Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E3-230

R&D Systems, part of Bio-Techne
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E3-230-050
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Q05086.4

Conjugate

Unconjugated

View all E6AP/UBE3A Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human E6AP/UBE3A protein
Met1 - Leu875 with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

102 kDa

Reviewed Applications

Read 1 review rated 5 using E3-230 in the following applications:

Formulation, Preparation and Storage

E3-230
Formulation Supplied as a solution in HEPES, NaCl and TCEP.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: E6AP/UBE3A

Ubiquitin-protein ligase E3A, also known as E6AP, is an E3 ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several key substrates for UBE3A have been identified, including BMAL1, the PML tumor suppressor, PGR, and p53/TP53 suggesting a role for UBE3A in regulation of the circadian clock, tumor regulation, transcriptional coactivation of the progesterone receptors, and regulation of neoplastic progression of cells infected by high-risk human papilloma virus (1-4). Defects in activity are linked to Angelman syndrome, a neurodevelopmental disorder, as well as autism spectrum disorders implicating a role for UBE3A in regulation of neurobiological functions (3, 5, 6).

References

  1. Dhananjayan, S.C. et. al. (2006) Mol. Endocrinol. 20:2343.
  2. Louria-Hayon, I. et. al. (2009) Cell Death Differ. 16:1156.
  3. Martinez-Noel, G. et. al. (2012) Mol. Cell. Biol. 32:3095.
  4. Gossan, N.C. et. al. (2014) Nucleic Acids Res. 42:5765.
  5. Sadikovic, B. et. al. (2014) Hum. Mutat. 35:1407.
  6. Khatri, N. and H-Y Man. (2019) Front. Mol. Neurosci. 12:109.

Long Name

Human Papillomavirus E6-associated Protein

Alternate Names

ANCR, EPVE6AP, HPVE6A, UBE3A

Entrez Gene IDs

7337 (Human); 22215 (Mouse); 361585 (Rat)

Gene Symbol

UBE3A

UniProt

Q05086.4

Additional E6AP/UBE3A Products

Product Documents for Recombinant Human His6-E6AP/UBE3A Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human His6-E6AP/UBE3A Protein, CF

For research use only

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