Recombinant Human His8-USP7 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # E-519
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human USP7 protein
Met1 - Asn1102 with a N-terminal 8-His tag
Met1 - Asn1102 with a N-terminal 8-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
130 kDa
Activity
Recombinant Human His8-USP7 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His8-USP7 concentration of 1-5 nM.
Reviewed Applications
Read 3 reviews rated 4.7 using E-519 in the following applications:
Formulation, Preparation and Storage
E-519
| Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, EDTA, DTT, and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: USP7
Ubiquitin Specific Peptidase 7 (USP7), also known as Herpes Virus-associated Ubiquitin Specific Protease (HAUSP), is a widely expressed deubiquitinating enzyme belonging to the peptidase C19 family (1). It has a predicted molecular weight of 130 kDa (2). Human USP7 is 1102 amino acids (aa) in length and shares 99% aa sequence identity with the mouse and rat orthologs (2,3). USP7 consists of a cysteine peptidase core (aa 208-560) that is flanked by an N-terminal TRAF-like domain (aa 50-205) and two C-terminal protease-resistant domains (aa 622-801 and 885-1061) (2,3). USP7 can be phosphorylated at Ser18 and Ser963 and ubiquitinated at Lys869 (2,4). USP7 was initially identified as a p53-interacting protein that deubiquitinates p53, thereby stabilizing the protein and inducing p53-dependent cell growth arrest and apoptosis (5). USP7 also targets the p53 regulatory proteins MDM2, MDMX, and Daxx, the epigenetic regulator Histone 2B, and the transcription factor FoxO4 (4,6-10). Additionally, USP7 interacts with the HSV-1 immediate early protein ICP0, contributing to the stabilization and transactivation capability of ICP0 during HSV-1 infection (1,11,12).
References
- Everett, R.D. et al. (1997) EMBO J. 16:1519.
- Fernández-Montalván, A. et al. (2007) FEBS J. 274:4256.
- Holowaty, M.N. et al. (2003) J. Biol. Chem. 278:47753.
- Khoronenkova, S.V. et al. (2012) Mol. Cell 45:801.
- Li, M. et al. (2002) Nature 416:648.
- Brooks, C.L. & W. Gu (2004) Cell Cycle 3:895.
- Meulmeester, E. et al. (2005) Cell Cycle 4:1166.
- Tanga, J. et al. (2010) Biochem. Biophys. Res. Commun. 393:542.
- van der Knaap, J.A. et al. (2005) Mol. Cell 17:695.
- van der Horst, A. et al. (2006) Nat. Cell Biol. 8:1043.
- Everett, R.D. et al. (1999) J. Virol. 73:417.
- Boutell, C. et al. (2005) J. Virol. 79:12342.
Long Name
Ubiquitin Specific Protease 7
Alternate Names
HAUSP, TEF1
Gene Symbol
USP7
UniProt
Additional USP7 Products
Product Documents for Recombinant Human His8-USP7 Protein, CF
Product Specific Notices for Recombinant Human His8-USP7 Protein, CF
For research use only
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