Recombinant Human HS3ST4 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6085-ST

R&D Systems, part of Bio-Techne
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6085-ST-020
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Key Product Details

Source

CHO

Accession #

NP_006031

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 protein
Gly184-Lys456 with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

HHHHHHGGAV

Predicted Molecular Mass

32 kDa

SDS-PAGE

35-43 kDa, reducing conditions

Activity

Measured by its ability to transfer sulfate from PAPS to heparan sulfate.
The specific activity is >100 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6085-ST
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4

Heparan sulfate is a highly sulfated polysaccharide that can be found on the cell surface and within the extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at the 2-O position by heparan sulfate 2‑O sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at the N, 3-O, and 6-O positions by N‑deacetylase/N‑sulfotransferases (NDSTs), heparan sulfate 3‑O sulfotransferases (HS3STs) and heparan sulfate 6-O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST4 and HS3ST2 are brain specific and may participate in HS-dependent neurobiologic events (5). HS3ST4 can generate tetrasulfated heparan sulfate disaccharide, the most highly sulfated sugar found in biological samples (6, 7), and may have a role in assisting HSV-1 entry and spread (8). HS3ST4 is a Golgi resident type II membrane protein and has the longest proline rich stem region among all HS3STs (3, 5). The activity of the recombinant human CHST1 is measured using a PAP-specific phosphatase-coupled sulfotransferase assay (9).

References

  1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
  2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
  3. Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.
  4. Xu, D. et al. (2005) Biochem. J. 386:451.
  5. Lawrence, R. et al. (2007) Matrix Biol. 26:442.
  6. Mochizuki, H. et al. (2003) J. Biol. Chem. 278:26780.
  7. Wu, Z.L. et al. (2004) J. Biol. Chem. 279:1861.
  8. Tiwari,V. et al. (2005) Biochem. Biophys. Res. Commun. 338:930.
  9. Prather, B. et al. (2012) Anal. Biochem. 423:86.

Alternate Names

3-OST-4

Entrez Gene IDs

9951 (Human)

Gene Symbol

HS3ST4

UniProt

NP_006031

Additional Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Products

Product Documents for Recombinant Human HS3ST4 Protein, CF

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Product Specific Notices for Recombinant Human HS3ST4 Protein, CF

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