Recombinant Human Hyaluronidase 4/HYAL4 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 6904-GH
Key Product Details
Learn more about Fluorescent Glycan Labeling and Detection
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human Hyaluronidase 4/HYAL4 protein
Cys34-Pro462, with a C-terminal 6-His tag
Cys34-Pro462, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Cys34
Predicted Molecular Mass
49 kDa
SDS-PAGE
66-85 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze S-35 labeled bovine cartilage chondroitin sulfate.
<500 ng of rhHYAL4 is required for the 50% hydrolysis of 10 μg chondroitin sulfate, as measured under the described conditions.
<500 ng of rhHYAL4 is required for the 50% hydrolysis of 10 μg chondroitin sulfate, as measured under the described conditions.
Formulation, Preparation and Storage
6904-GH
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Hyaluronidase 4/HYAL4
Human hyaluronidases (HYALs) are a group of five endo-beta -N-acetyl-hexosaminidases that include HYAL1, HYAL2, HYAL3, HYAL4, and SPAM1 (PH20) (1, 2). While HYAL1, HYAL2 and SPAM1 are endo-beta -N-acetyl-glucosaminidase that are mainly active on hyaluronan with little activity on chondroitin sulfate (3, 4, 5), HYAL4 is the only known endo-beta -N-acetyl-galactosaminidase that is mainly active on chondroitin sulfate (type C and D in particular) with no activity on hyaluronan (6). Hyaluronan and chondroitin sulfate are abundant extracellular matrix components that have numerous biological functions (7). The backbone of chondroitin sulfate composed of repeating units of‑4GlcA beta‑3GalNAc beta1‑ most closely resembles to that of hyaluronan composed of repeating units of ‑4GlcA beta‑3GlcNAc beta1‑, which may partially explain the overlapping substrate specificity of most hyaluronidases. HYAL4 is believed to specifically degrade chondroitin sulfate in the lysosome (8), as its optimal pH for activity is around 4.5 (6). Accordingly, the enzymatic activity was measured by treating 35S-labeled chondroitin sulfate with recombinant HYAL4 (9). HYAL4 may be used to investigate the biological roles of chondroitin sulfate in tissues and cells.
References
- Csoka, A.B. et al. (2001) Matrix Biol. 20:499.
- Jedrzejas, M. J. and Stern, R. Proteins (2005) 61:227.
- Csoka, A.B. et al. (1999) Genomics 60:351.
- Lin, Y. et al. (1994) J. Cell. Biol. 125:1157.
- Lepperdinger, G. et al. (2001) Matrix Biol. 20:509.
- Kaneiwa, T. et al. (2010) Glycobiology 20:300.
- Iozzo, R.V. (1998) Annu. Rev. Biochem. 67:609.
- Prabhakar, v. and Sasisekharan, R. (2006) Adv. Pharmacol. 53:69.
- Ethen, C.M. et al. http://www.rndsystems.com/resources/images/21388.pdf
Long Name
Hyaluronoglucosaminidase-4
Alternate Names
CSHY, HYAL4
Gene Symbol
HYAL4
UniProt
Additional Hyaluronidase 4/HYAL4 Products
Product Documents for Recombinant Human Hyaluronidase 4/HYAL4 Protein, CF
Product Specific Notices for Recombinant Human Hyaluronidase 4/HYAL4 Protein, CF
For research use only
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