Recombinant Human ICAM-4 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10407-IC

R&D Systems, part of Bio-Techne
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Key Product Details

Source

CHO

Accession #

Q14773.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all ICAM-4 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ICAM-4 protein
Human ICAM-4
(Ala31-Ala240)
Accession # Q14773.1		 DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala31

Predicted Molecular Mass

50 kDa

SDS-PAGE

50-60 kDa & 70-80 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Integrin alphaL beta2  (Catalog # 3868-AV) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human ICAM-4 Fc Chimera (Catalog # 10407-IC) binds with an ED50 of 0.25-1.5 μg/mL.

Scientific Data Images for Recombinant Human ICAM-4 Fc Chimera Protein, CF

Recombinant Human ICAM-4 Fc Chimera Protein Binding Activity

Recombinant Human ICAM-4 Fc Chimera Protein Binding Activity

When Recombinant Human Integrin alpha L beta 2 Protein (3868-AV) is immobilized at 1 μg/mL (100 μL/well), Recombinant Human ICAM-4 Fc Chimera (10407-IC) binds with an ED50 of 0.25-1.5 μg/mL.
Recombinant Human ICAM-4 Fc Chimera Protein SDS-PAGE

Recombinant Human ICAM-4 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human ICAM-4 Fc Chimera (Catalog # 10407-IC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 50-60 kDa & 70-80 kDa, and 140-160 kDa, respectively.

Formulation, Preparation and Storage

10407-IC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ICAM-4

ICAM-4 (intercellular adhesion molecule-4), also known as CD242, is a transmembrane cell adhesion glycoprotein and a member of the immunoglobulin protein superfamily. The ICAM sub-family consists of five members, ICAM-1 through ICAM-5, and they vary in their tissue expression and number of Ig-like domains in the extracellular domain (ECD) (1). Full-length human ICAM-4 contains 2 Ig-like domains in the ECD, a single transmembrane domain and short intracellular domain. Alternative splicing of ICAM-4 results in at least one soluble form (2). The ECD of mature human ICAM-4 shares 68% and 67% amino acid sequence identity with mouse and rat ICAM-4, respectively. ICAM-4 expression is limited to erythroid and possibly placental tissue but its biological role remains poorly defined (3). ICAM-4 has been shown to bind alpha4 beta1 and alphaV family integrins as well as displaying broad ligand binding specificity for some beta1, beta2, beta3 and beta5 integrins (4, 5). ICAM-4 binding to endothelial alphav beta3 has been indicated as a factor in vaso-occlusion, particularly in sickle cell disease (6). ICAM-4 is expressed on red blood cells (RBC), erythroid precursor cells, and possibly placental tissue (3, 7). ICAM-4 has shown to bind alpha4 beta1 on hemopoietic cells, alphaV family integrins (avb1, avb3, and avb5) on non-hemopoietic cells as well as displaying broad ligand binding specificity for some beta1, beta2, beta3 and beta5 integrins (4, 5, 7). Studies have shown aLb2 integrin interacts through the first Ig-like domain of ICAM-4, whereas aMb2 and aXb2 integrins interact through both Ig-like domains of ICAM-4 (7). In addition, initiation of vaso-occlusion in sickle cell disease is implicated by ICAM-4 binding to endothelial alphav beta3 integrin (6). The ability of ICAM-4 to interact selectively with different integrins suggests its importance in RBC physiology and pathology as well as its therapeutic value.

References

  1. Gahmberg, C.G. et al. (1997) Eur. J. Biochem. 245, 215.
  2. Lee, G. et al. (2003) Blood 101:1790.
  3. Southcott, M.J.G. et al. (1999) Blood 93:4425.
  4. Spring, F.A. et al. (2001) Blood. 98:458.
  5. Hermand, P. et al. (2003) J Biol Chem. 278:4892.
  6. Kaul, D.K. et al. (2006) Am J Physiol Cell Physiol. 291:C922.
  7. Ihanus, E. et al. (2007) Blood 109:802.

Long Name

Intercellular Adhesion Molecule 4

Alternate Names

CD242, ICAM4

Entrez Gene IDs

3386 (Human); 78369 (Mouse)

Gene Symbol

ICAM4

UniProt

Q14773.1

Additional ICAM-4 Products

Product Documents for Recombinant Human ICAM-4 Fc Chimera Protein, CF

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