Recombinant Human ICOS Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 169-CS
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human ICOS protein
| Human ICOS (Glu21-Phe141) Accession # Q9Y6W8 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Glu21
Predicted Molecular Mass
40.3 kDa (monomer)
SDS-PAGE
50-60 kDa, reducing conditions
Activity
Measured by its ability to inhibit human T cell proliferation induced by rhB7-H2/Fc Chimera in the presence of anti-CD3. Wang, S. et al. (2000) Blood 96:2808.
The ED50 for this effect is 1-4 µg/mL in the presence of 3 µg/mL Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) and at least 20 ng/mL of anti-CD3.
Optimal dilutions should be determined by each laboratory for each application.
The ED50 for this effect is 1-4 µg/mL in the presence of 3 µg/mL Recombinant Human B7‑H2 Fc Chimera (Catalog # 165-B7) and at least 20 ng/mL of anti-CD3.
Optimal dilutions should be determined by each laboratory for each application.
Reviewed Applications
Read 7 reviews rated 4.7 using 169-CS in the following applications:
Scientific Data Images for Recombinant Human ICOS Fc Chimera Protein, CF
Recombinant Human ICOS Fc Chimera Protein Bioactivity
Recombinant Human ICOS Fc Chimera (Catalog # 169-CS) inhibits rhB7-H2/Fc Chimera (Catalog # 165-B7) induced human T cell proliferation in the presence of anti-CD3. The ED50 for this effect is 1-4 µg/mL.Formulation, Preparation and Storage
169-CS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: ICOS
Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% amino acid similarity with CD28 and CTLA-4. Human and mouse ICOS share approximately 72% amino acid identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within approximately 24 - 48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.
References
- Aicher, A. et al. (2000) J. Immunol. 164:4689.
- Coyle, A.J. et al. (2000) Immunity 13:95.
- Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
- Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
- Hutloff, A. et al. (1999) Nature 397:263.
- Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
- Yoshinaga, S.K. et al. (1999) Nature 402:827.
Long Name
Inducible Co-stimulator
Alternate Names
AILIM, CD278, CRP-1
Gene Symbol
ICOS
UniProt
Additional ICOS Products
Product Documents for Recombinant Human ICOS Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human ICOS Fc Chimera Protein, CF
For research use only
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