Recombinant Human IDO2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9967-AO

Indoleamine 2,3-dioxygenase 2
R&D Systems, part of Bio-Techne
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9967-AO-050
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Key Product Details

Source

E. coli

Accession #

Q6ZQW0-1

Conjugate

Unconjugated

Applications

Enzyme Activity

View all IDO2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IDO2 protein
Glu15-Gly420, with an N-terminal Met and C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

46 kDa

SDS-PAGE

41 kDa, reducing conditions

Activity

Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine.
The specific activity is >15 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant Human IDO2 Protein, CF

Recombinant Human IDO2 Protein Enzyme Activity

Recombinant Human IDO2 Protein Enzyme Activity

Recombinant Human IDO2 (Catalog # 9967-AO) is measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine. The activity (orange) is approximately 4-fold greater than the competitor's IDO2 (green).
Recombinant Human IDO2 Protein SDS-PAGE

Recombinant Human IDO2 Protein SDS-PAGE

1 μg/lane of Recombinant Human IDO2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silverstaining, showing a band at 41 kDa.

Formulation, Preparation and Storage

9967-AO
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: IDO2

Indoleamine 2,3-dioxygenase (IDO2) is a 47 kDa heme-containing cytosolic dioxygenase. Human IDO2 shares 64% aa sequence identity with mouse IDO2. IDO2 is one of three dioxygenases capable of catalyzing the first and rate-limiting step of the L-kynurenine pathway (KP): oxidative cleavage of the essential amino acid L-tryptophan to form N formyl kynurenine (1). Of these proteins, IDO1 and IDO2 are both related, monomeric enzymes but share only 38% aa sequence identity. The IDO isoforms are not functionally redundant. Although expression of IDO2 has been upregulated in some cancers (2,3), IDO2 expression is generally restricted to the liver, kidney, brain, and certain immune cell types unlike the more ubiquitously expressed indoleamine 2,3-dioxygenase (IDO) (1). Differential inhibition of IDO1 and IDO2 is observed with several molecules (4,5). IDO2 has significantly lower tryptophan catabolic activity than IDO1 and TDO2 (4, 6-8) suggesting it does not play a significant physiological role in the KP. Instead, IDO2 may have an alternative functional role: either non-enzymatic or utilizing a more physiologically relevant substrate (5,7).  IDO2 function operates as a pro-inflammatory mediator in autoimmune inflammatory disorders (8, 9-11). It is a candidate for co-therapeutic targeting for treatment in these diseases (10-11).

References

  1. Prendergast, G.C. et al. (2014) Front. Immunol. 5:585.
  2. Witkiewicz, A.K. et al. (2009) J. Am. Coll. Surg. 208:781.
  3. Lob, S. et al. (2009) Cancer Immunol. Immunother. 58:153.
  4. Meininger, D. et al. (2011) Biochim. Biophys. Acta. 1814:1947.
  5. Pantouris, G. et al. (2014) Amino Acids 46:2155.
  6. Ball, H.J. et al. (2007) Gene 396:203.
  7. Yuasa, H.J. et al. (2009) Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 153:137.
  8. Metz, R. et al. (2014) Int. Immunol. 26:357.
  9. Merlo, L.M.F. et al. (2014) J. Immunol. 192:2082.
  10. Merlo, L.M. and L. Mandik-Nayak (2016) Clin. Med. Insights Pathol. 9:21.
  11. Merlo, L.M.F. et al (2017) Clin. Immunol. 179:8.

Long Name

Indoleamine 2,3-dioxygenase 2

Alternate Names

Ido-2, INDOL1, Indoleamine 2,3-dioxygenase-2

Entrez Gene IDs

169355 (Human); 209176 (Mouse); 681319 (Rat)

Gene Symbol

IDO2

UniProt

Q6ZQW0-1

Additional IDO2 Products

Product Documents for Recombinant Human IDO2 Protein, CF

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Product Specific Notices for Recombinant Human IDO2 Protein, CF

For research use only

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