Recombinant Human IFN-alpha/beta R1 Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11574

Fc Chimera
R&D Systems, part of Bio-Techne
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AVI11574-050
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Key Product Details

Source

CHO

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IFN-alpha/beta R1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IFN-alpha/beta R1 protein
Human IFN-A/B R1
(Gly26-Lys436)
Accession # AAA52730.1		 GGIEGRMD Human IgG1 Fc
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly26

Predicted Molecular Mass

76 kDa

SDS-PAGE

95-125 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human IFN-alpha / beta R1 Fc Chimera Avi-tag (Catalog # AVI11574) binds Recombinant Human IFN-alpha / beta R2 Fc Chimera (Catalog # 4015-AB) in the presence of Recombinant Human IFN-beta (Catalog # 8499-IF) with an ED50 of 5.00-60.0 ng/mL.

Scientific Data Images for Recombinant Human IFN-alpha/beta R1 Fc Avi-tag Protein, CF

Biotinylated Recombinant Human IFN‑ alpha/ beta R1 Fc Chimera Avi-tag Protein Binding Activity.

Biotinylated Recombinant Human IFN-alpha / beta R1 Fc Chimera Avi-tag Protein (Catalog # AVI11574) binds Recombinant Human IFN-alpha / beta R2 Fc Chimera (4015-AB) in the presence of Recombinant Human IFN-beta (8499-IF) with an ED50 of 5.00-60.0 ng/mL.

Biotinylated Recombinant Human IFN‑ alpha/ beta R1 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human IFN‑ alpha/ beta R1 Fc Chimera Avi-tag Protein (Catalog # AVI11574) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-125 kDa and 190-250 kDa, respectively.

Formulation, Preparation and Storage

AVI11574
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha/beta R1

Interferon‑alpha/beta receptor 1 (IFN‑ alpha/ beta R1), also known as IFNAR1, is a 100‑130 kDa member of the class II cytokine receptor family of proteins. These proteins form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1). IFN‑ alpha/ beta R1, in association with IFN‑ alpha/ beta R2, is required for propagating anti‑microbial signal transduction triggered by the type 1 interferons such as IFN‑ alpha and IFN‑ beta (2, 3). Mature human IFN‑ alpha/ beta R1 consists of a 409 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 100 aa cytoplasmic domain (4). The ECD contains three tandem fibronectin type III repeats and is extensively glycosylated. Within the ECD, human IFN‑ alpha/ beta R1 shares 47% and 50% aa identity with mouse and rat  IFN‑ alpha/ beta R1, respectively. Alternative splicing generates two additional isoforms that lack the transmembrane segment and either all or a portion of the cytoplasmic domain. IFN‑ alpha/ beta R1 interacts very weakly or not at all with type 1 interferons and does not stably interact with IFN‑ alpha/ beta R2. Ligands preferentially associate with IFN‑ alpha/ beta R2, and this complex subsequently forms a stable ternary assembly with IFN‑ alpha/ beta R1 (5‑7). IFN‑ alpha/ beta R1 also associates with IFN‑ gamma R2 even in the absence of IFN‑ gamma stimulation (3). IFN‑ alpha/ beta R1 activation depends on tyrosine phoshorylation as well as palmitoylation of its cytoplasmic domain (8, 9). Rapid down‑regulation of the receptor is accomplished by ligand‑dependent or ‑independent pathways (e.g. VEGF R signaling, TLR signaling, or cellular stress) which induce its serine phosphorylation, ubiquitination, and degradation (10‑13).  Our Avi-tag Biotinylated human IFN‑ alpha/ beta R1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
  2. Hwang, S.Y. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11284.
  3. Takaoka, A. et al. (2000) Science 288:2357.
  4. Uze, G. et al. (1990) Cell 60:225.
  5. Lamken, P. et al. (2004) J. Mol. Biol. 341:303.
  6. Arduini, R.M. et al. (1999) Prot. Sci. 8:1867.
  7. Kalie, E. et al. (2008) J. Biol. Chem. 283:32925.
  8. Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
  9. Claudinon, J. et al. (2009) J. Biol. Chem. 284:24328.
  10. Zheng, H. et al. (2011) Blood 118:4003.
  11. Qian, J. et al. (2011) PLoS Pathogens 7:e1002065.
  12. Bhattacharya, S. et al. (2010) J. Biol. Chem. 285:2318.
  13. Bhattacharya, S. et al. (2011) J. Biol. Chem. 286:22069.

Long Name

Interferon alpha/beta Receptor 1

Alternate Names

IFN-aR1, IFN-bR1, IFNAR1, IFNbR1

Entrez Gene IDs

3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)

Gene Symbol

IFNAR1

Additional IFN-alpha/beta R1 Products

Product Documents for Recombinant Human IFN-alpha/beta R1 Fc Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IFN-alpha/beta R1 Fc Avi-tag Protein, CF

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