Recombinant Human IFN-alpha H2/IFNA14 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 11019-IF
Key Product Details
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived IFN-alpha H2/IFNA14 protein
Cys24-Asp189
Cys24-Asp189
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Cys24
Predicted Molecular Mass
20 kDa
SDS-PAGE
22-26 kDa, under reducing conditions.
Activity
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129.
The ED50 for this effect is 1.50-15.0 pg/mL.
The ED50 for this effect is 1.50-15.0 pg/mL.
Scientific Data Images for Recombinant Human IFN-alpha H2/IFNA14 Protein, CF
Recombinant Human IFN-alpha H2/IFNA14 Protein Binding Activity.
Recombinant Human IFN-alpha H2/IFNA14 (Catalog # 11019-IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 1.50-15.0 pg/mLRecombinant Human IFN-alpha H2/IFNA14 Protein SDS-PAGE.
1 μg/lane of Recombinant Human IFN-alpha H2/IFNA14 Protein (Catalog # 11019-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 22-26 kDa, respectively.Formulation, Preparation and Storage
11019-IF
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
Reconstitution | Reconstitute at 100 μg/mL in PBS. |
Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: IFN-alpha H2/IFNA14
References
- Pestka, S. et al. (1987) Annu Rev Biochem. 56:727.
- Goeddel, D.V. et al. (1980) Nature 287:411.
- Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
- Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139.
- Wittling, M.C. et al. (2021) Front Immunol. 11:605673.
- van Pesch, V. et al. (2004) J. Virol. 78:8219.
- James, C.M. et al. (2007) Vaccine. 25(10):1856.
- Moll, H.P. et al. (2011) Cytokine. 53:52.
- Lavender, K.J. et al. (2016) J Virol. 90:6001.
Long Name
Interferon alpha H2
Alternate Names
IFN-alphaH, IFNA14, IFNalpha H2, LEIF2H
Gene Symbol
IFNA14
UniProt
Additional IFN-alpha H2/IFNA14 Products
Product Documents for Recombinant Human IFN-alpha H2/IFNA14 Protein, CF
Product Specific Notices for Recombinant Human IFN-alpha H2/IFNA14 Protein, CF
For research use only
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