Recombinant Human IFN-epsilon Protein

R&D Systems, part of Bio-Techne | Catalog # 9667-ME

R&D Systems, part of Bio-Techne
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Carrier Free
9667-ME-025/CF
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With Carrier
9667-ME-025
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Key Product Details

Source

E. coli

Accession #

Q86WN2

Conjugate

Unconjugated

Applications

Bioactivity

View all IFN-epsilon Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IFN-epsilon protein
Leu22-Arg208 (Cys175Ser), with an N-terminal Met and a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

23 kDa

SDS-PAGE

23 kDa, reducing conditions

Activity

Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129.
The ED50 for this effect is 100-500 ng/mL.

Reviewed Applications

Read 1 review rated 4 using 9667-ME in the following applications:

Scientific Data Images for Recombinant Human IFN-epsilon Protein

Recombinant Human IFN-epsilon Protein Bioactivity

Recombinant Human IFN-epsilon Protein Bioactivity

Recombinant Human IFN-epsilon (Catalog # 9667-ME) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 100‑500 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 9667-ME
Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 9667-ME/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Citric Acid and CHAPS.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-epsilon

Interferon-epsilon (IFN-epsilon) is a secreted, approximately 23 kDa member of the type I interferon family of molecules (1). Mature human IFN-epsilon shares 62% and 60% amino acid sequence identity with rat and mouse IFN-epsilon, respectively. IFN-epsilon signals through IFN‑alpha / beta R1 and IFN‑alpha / beta R2 and contributes to TNF-alpha induced signaling (2, 3). It is constitutively expressed in epithelial cells lining the lung, intestines, testes, and female reproductive tract, and it is further up-regulated in the uterus by estrogen (2-6). IFN-epsilon provides mucosal barrier protection against the pathogens Chlamidia and Herpes simplex virus 2 (2). It induces B cell activation, attraction of CD8+ T cells to sites of virus infection and directly mediates protection against viral and bacterial genital infections (5, 7). Differential regulation of genes related to central nervous system by recombinant human IFN-epsilon suggests its role in maintenance of the structure and function of brain (8).

References

  1. Wijesundara, D.K. et al. (2014) Front. Immunol. 5:412.
  2. Fung, K.Y. et al. (2013) Science 339:1088.
  3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  4. Demers, A. et al. (2014) J. Leukoc. Biol. 96:1101.
  5. Xi, Y. et al. (2012) Mucosal Immunol. 5:610.
  6. Hermant, P. et al. (2013) PLoS One 8:e71320.
  7. Day, S.L. et al. (2008) J. Immunol. 180:7158.
  8. Peng, Fu-Wang et.al. (2007) Protein Expression and Purification 53:356.

Long Name

Interferon epsilon

Alternate Names

Ifn-tau-11, IFNE, IFNepsilon, IFNT1, INFE1

Entrez Gene IDs

338376 (Human); 230405 (Mouse); 298225 (Rat)

Gene Symbol

IFNE

UniProt

Q86WN2

Additional IFN-epsilon Products

Product Documents for Recombinant Human IFN-epsilon Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human IFN-epsilon Protein

For research use only

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