Recombinant Human IFN-gamma R1/CD119 Avi-tag His Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11030

Biotinylated
R&D Systems, part of Bio-Techne
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AVI11030-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P15260.1

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IFN-gamma R1/CD119 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IFN-gamma R1/CD119 protein
Human IFNGR1
(Glu18-Gly245)
Accession # P15260.1		 Avi-tag 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu18 and Gly20

Predicted Molecular Mass

29 kDa

SDS-PAGE

42-55 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinan human IFN-gamma (Catalog # 285-IF/CF) is immobilized at 2.00 µg/mL (100 µL/well), In the presence of Recombinant Human IFN-gamma R2, Biotinylated Recombinant Human IFN-gamma R1/CD119 Avi-tag His-tag binds with an ED50 of 0.750-4.50 µg/mL.

Scientific Data Images for Recombinant Human IFN-gamma R1/CD119 Avi-tag His Protein, CF

Biotinylated Recombinant Human IFN‑ gamma R1/CD119 Avi-tag His-tag Protein Binding Activity.

When Recombinant human IFN-gamma (285-IF/CF) is immobilized at 2.00 µg/mL (100 µL/well), In the presence of Recombinant Human IFN-gamma R2, Biotinylated Recombinant Human IFN-gamma R1/CD119 Avi-tag His-tag (Catalog # AVI11030) binds with an ED50 of 0.750-4.50 µg/mL.

Biotinylated Recombinant Human IFN‑ gamma R1/CD119 Avi-tag His-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human IFN‑ gamma R1/CD119 Avi-tag His-tag Protein (Catalog # AVI11030) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 42-55 kDa.

Formulation, Preparation and Storage

AVI11030
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma R1/CD119

Interferon gamma receptor 1 (IFNGR1), along with IFNGR2, are type II cytokine receptors that combine to form a high affinity signaling complex with the type II interferon, IFNG. Mature human IFNGR1 consists of an extracellular domain (ECD) with 2 Ig-like domains, a transmembrane domain and an intracellular domain with both Jak1 and Stat1 binding motifs. The ECD of human IFNGR1 shares 50% amino acid sequence identity with mouse IFNGR1. The IFNG signaling complex is formed by 2 IFNGR1 subunits binding one IFNG dimer directly, and then 2 IFNGR2 molecules further stabilizing the receptor complex. Complex formation then triggers a signaling cascade that culminates in the transcription of the interferon stimulated genes (ISGs) and additional transcription factors. Ultimately, IFNGR1 mediated signaling regulates several biological processes including innate and acquired immune response, apoptosis and cell cycle progression. IFNGR1 is constitutively expressed in most cell types and deletions or mutations to IFNGR1 result in reduced resistance to bacterial, parasitic, and viral infection. Our Avi-tag Biotinylated IFNGR1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Bhat, M.Y. et al. (2018) J. Cell Commun Signal 12:745.
  2. de Weerd, N.A. and Nguyen, T. (2012) Immunol Cell Biol. 90:483.
  3. Mendoza, J.L. et al. (2019) Nature 567:56.
  4. Blouin, C.M. and Lamaze, C. (2013) Front Immunol. 4:267.
  5. Alspach, E. et al. (2019) Cold Spring Harb Perspect Biol. 11:a028480.

Long Name

Interferon gamma Receptor 1

Alternate Names

CD119, IFN-gR1, IFNgammaR1, IFNGR1

Entrez Gene IDs

3459 (Human); 15979 (Mouse)

Gene Symbol

IFNGR1

UniProt

P15260.1

Additional IFN-gamma R1/CD119 Products

Product Documents for Recombinant Human IFN-gamma R1/CD119 Avi-tag His Protein, CF

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Product Specific Notices for Recombinant Human IFN-gamma R1/CD119 Avi-tag His Protein, CF

For research use only

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