Recombinant Human IL-1 RAcP/IL-1 R3 Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11139

Biotinylated Fc Chimera
R&D Systems, part of Bio-Techne
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AVI11139-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

Q9NPH3.2

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IL-1 RAcP/IL-1 R3 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-1 RAcP/IL-1 R3 protein
Human IL-1RAP
(Ser21-Glu359)
Accession # Q9NPH3.2		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser21

Predicted Molecular Mass

68 kDa

SDS-PAGE

83 - 93 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.

When Recombinant Human IL-1 RII Fc Chimera (Catalog # 663-2R) is immobilized at 0.5 µg/mL (100 µL/well), in the presence of Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB), it binds to Biotinylated Recombinant Human IL-1 RAcP/IL-1 R3 Fc Chimera Avi-tag (Catalog # AVI11139) with an ED50 of 4.00-36.0 ng/mL.

Scientific Data Images for Recombinant Human IL-1 RAcP/IL-1 R3 Fc Avi-tag Protein, CF

Biotinylated Recombinant Human IL‑1 RAcP/IL‑1 R3 Fc Chimera Avi-tag Protein Binding Activity.

When Recombinant Human IL-1 RII Fc Chimera (663-2R) is immobilized at 0.5 µg/mL (100 µL/well), in the presence of Recombinant Human IL-1 beta/IL-1F2 (201-LB), it binds to Biotinylated Recombinant Human IL-1 RAcP/IL-1 R3 Fc Chimera Avi-tag Protein (Catalog # AVI11139) with an ED50 of 4.00-36.0 ng/mL.

Biotinylated Recombinant Human IL‑1 RAcP/IL‑1 R3 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human IL‑1 RAcP/IL‑1 R3 Fc Chimera Avi-tag Protein (Catalog # AVI11139) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 83-93 kDa and 170-190 kDa, respectively.

Formulation, Preparation and Storage

AVI11139
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 RAcP/IL-1 R3

IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins (1). It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36 (2-4). It is a subunit of the functional signaling complex with IL-1 RI and associates with IL-1 RII in a non-signaling receptor complex (2, 5). In addition, it interacts with ST2/IL-1 R4 on mast cells and Th2 cells to create a functional IL-33 receptor complex (3). IL-1 RAcP also functions as a co-receptor for IL-36 alpha/IL-1F6, IL-36 beta/IL-1F8, and IL-36 gamma/IL-1F9 (4). Mature human IL-1 RAcP consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 182 aa cytoplasmic domain (2). Within the ECD, human IL-1 RAcP shares 86% aa sequence identity with mouse and rat IL-1 RAcP. Alternative splicing generates two secreted decoy receptor isoforms and an isoform with a substituted cytoplasmic domain (6-8). When present with soluble IL-1 RII, soluble IL-1 RAcP increases the IL-1 binding affinity of IL-1 RII more than 100-fold, thus neutralizing the effects of IL-1 (Our Avi-tag Biotinylated human IL-1 RAcP Fc chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Boraschi, D. and A. Tagliabue (2013) Semin. Immunol. 25:394.
  2. Greenfeder, S.A. et al. (1995) J. Biol. Chem. 270:13757.
  3. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  4. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  5. Lang, D. et al. (1998) J. Immunol. 161:6871.
  6. Lu, H.-L. et al. (2008) Mol. Immunol. 45:1374.
  7. Jensen, L.E. et al. (2000) J. Immunol. 164:5277.
  8. Jensen, L.E. and A.S. Whitehead (2003) Cell. Signal. 15:793.
  9. Smith, D.E. et al. (2003) Immunity 18:87.
  10. Yoshida, T. et al. (2012) J. Neurosci. 32:2588.

Long Name

Interleukin 1 Receptor Accessory Protein

Alternate Names

IL-1 R3, IL-1RAcP, IL1RAcP, IL1RAP

Entrez Gene IDs

3556 (Human); 16180 (Mouse)

Gene Symbol

IL1RAP

UniProt

Q9NPH3.2

Additional IL-1 RAcP/IL-1 R3 Products

Product Documents for Recombinant Human IL-1 RAcP/IL-1 R3 Fc Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human IL-1 RAcP/IL-1 R3 Fc Avi-tag Protein, CF

For research use only

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