Recombinant Human IL-1 RAcP/IL-1 R3 His Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9176

Biotinylated
R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
AVI9176-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

CHO

Accession #

Q9NPH3.2

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IL-1 RAcP/IL-1 R3 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-1 RAcP/IL-1 R3 protein
Human IL-1 RAcP/IL-1 R3
(Ser21-Glu359)
Accession # Q9NPH3.2		 6-His tag Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser21

Predicted Molecular Mass

42 kDa

SDS-PAGE

59-65 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-1 RI Fc Chimera (Catalog # 11085-RI) is immobilized at 2 µg/mL (100 µL/well) in the presence of Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB) , it binds to Biotinylated Recombinant Human IL-1 RAcP/IL-1 R3 His-tag Avi-tag (Catalog # AVI9176) with an ED50 of 50.0-400 ng/mL.

Scientific Data Images for Recombinant Human IL-1 RAcP/IL-1 R3 His Avi-tag Protein, CF

Recombinant Human IL-1 RAcP/IL-1 R3 His-tag Avi-tag Protein Binding Activity.

When Recombinant Human IL‑1 RI Fc Chimera Protein (11085-RI) is immobilized at 2 µg/mL (100 µL/well) in the presence of Recombinant Human IL‑1 beta/IL‑1F2 Protein (201-LB), it binds to Biotinylated Recombinant Human IL‑1 RAcP/IL‑1 R3 His-tag Avi-tag Protein (Catalog # AVI9176) with an ED50 of 50.0-400 ng/mL.

Recombinant Human IL-1 RAcP/IL-1 R3 His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL-1 RAcP/IL-1 R3 His-tag Avi-tag Protein (Catalog # AVI9176) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 59-65 kDa.

Formulation, Preparation and Storage

AVI9176
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 RAcP/IL-1 R3

IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins (1). It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36 (2-4). It is a subunit of the functional signaling complex with IL-1 RI and associates with IL-1 RII in a non-signaling receptor complex (2, 5). In addition, it interacts with ST2/IL-1 R4 on mast cells and Th2 cells to create a functional IL-33 receptor complex (3). IL-1 RAcP also functions as a co-receptor for IL-36 alpha/IL-1F6, IL-36 beta/IL-1F8, and IL-36 gamma/IL-1F9 (4). Mature human IL-1 RAcP consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 21 aa transmembrane segment, and a 182 aa cytoplasmic domain (2). Within the ECD, human IL-1 RAcP shares 86% aa sequence identity with mouse and rat IL-1 RAcP. Alternative splicing generates two secreted decoy receptor isoforms and an isoform with a substituted cytoplasmic domain (6-8). When present with soluble IL-1 RII, soluble IL-1 RAcP increases the IL-1 binding affinity of IL-1 RII more than 100-fold, thus neutralizing the effects of IL-1 (9). Neuronal IL-1 RAcP interacts trans-synaptically with PTP sigma and can induce excitatory pre- and post-synaptic development (10). . Our Avi-tag Biotinylated Human IL-1 RAcP features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Boraschi, D. and A. Tagliabue (2013) Semin. Immunol. 25:394.
  2. Greenfeder, S.A. et al. (1995) J. Biol. Chem. 270:13757.
  3. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  4. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
  5. Lang, D. et al. (1998) J. Immunol. 161:6871.
  6. Lu, H.-L. et al. (2008) Mol. Immunol. 45:1374.
  7. Jensen, L.E. et al. (2000) J. Immunol. 164:5277.
  8. Jensen, L.E. and A.S. Whitehead (2003) Cell. Signal. 15:793.
  9. Smith, D.E. et al. (2003) Immunity 18:87.
  10. Yoshida, T. et al. (2012) J. Neurosci. 32:2588.

Long Name

Interleukin 1 Receptor Accessory Protein

Alternate Names

IL-1 R3, IL-1RAcP, IL1RAcP, IL1RAP

Entrez Gene IDs

3556 (Human); 16180 (Mouse)

Gene Symbol

IL1RAP

UniProt

Q9NPH3.2

Additional IL-1 RAcP/IL-1 R3 Products

Product Documents for Recombinant Human IL-1 RAcP/IL-1 R3 His Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human IL-1 RAcP/IL-1 R3 His Avi-tag Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.