Recombinant Human IL-10 R alpha Avi-tag His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11459

R&D Systems, part of Bio-Techne
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AVI11459-050
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Key Product Details

Source

CHO

Accession #

Q13651.2

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IL-10 R alpha Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-10 R alpha protein
Human IL-10 R alpha
(His22-Asn235)
Accession # Q13651.2		 Avi-tag 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His22

Predicted Molecular Mass

28 kDa

SDS-PAGE

40-55 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human IL-10 R alpha Avi-tag His-tag (Catalog # AVI11459) binds Recombinant Human IL-10 (Catalog # 1064-ILB) with an ED50 of 8.00-96.0 ng/mL.

Scientific Data Images for Recombinant Human IL-10 R alpha Avi-tag His-tag Protein, CF

Recombinant Human IL‑10 R alpha Avi-tag His-tag Protein Binding Activity.

Recombinant Human IL‑10 R alpha Avi-tag His-tag Protein (Catalog # AVI11459) binds Recombinant Human IL‑10 (1064-ILB) with an ED50 of 8.00-96.0 ng/mL.

Recombinant Human IL‑10 R alpha Avi-tag His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL‑10 R alpha Avi-tag His-tag Protein (Catalog # AVI11459) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 40-55 kDa, under reducing conditions.

Formulation, Preparation and Storage

AVI11459
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10 R alpha

Interleukin-10 Receptor alpha (IL-10 R alpha), also known as IL-10 R1, is a 90-110 kDa transmembrane glycoprotein member of the class II cytokine receptor family (1). IL-10 R alpha is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer (2-5). Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6). IL-10 R alpha is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL-10 R alpha, resulting in the recruitment of IL-10 R beta (6-8). IL-10 R beta is a ubiquitously expressed transmembrane protein that does not bind IL-10 by itself but is required for signal transduction and in vivo IL-10 responsiveness (7, 9). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (1). Immunosuppressive signal transduction through the IL-10 receptor complex can be inhibited by activation of TLR2, 4, or 9, enabling strengthened immune responses during infection (10). Polymorphisms of human IL-10 R alpha may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL-10 (11). Mature human IL-10 R alpha consists of a 214 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 322 aa cytoplasmic domain (12). Within the ECD, human IL-10 R alpha shares 59% aa sequence identity with mouse and rat IL-10 R alpha. Our Avi-tag Biotinylated human IL-10R alpha features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. Manzanillo, P. et al. (2015) Trends Immunol. 36:471.
  3. Sziksz, E. et al. (2015) Mediators Inflamm. 2015:764641.
  4. Mannino, M.H. et al. (2015) Cancer Lett. 367:103.
  5. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  6. Tan, J.C. et al. (1993) J. Biol. Chem. 268:21053.
  7. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  8. Tan, J.C. et al. (1995) J. Biol. Chem. 270:12906.
  9. Spencer, S.D. et al. (1998) J. Exp. Med. 187:571.
  10. Fernandez, S. et al. (2004) J. Immunol. 172:2613.
  11. Gruber, S.G. et al. (2008) Eur. J. Immunol. 38:3365.
  12. Liu, Y. et al. (1994) J. Immunol. 152:1821.

Long Name

Interleukin 10 Receptor alpha

Alternate Names

CDw210a, IL-10Ra, IL10R alpha, IL10RA

Entrez Gene IDs

3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)

Gene Symbol

IL10RA

UniProt

Q13651.2

Additional IL-10 R alpha Products

Product Documents for Recombinant Human IL-10 R alpha Avi-tag His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-10 R alpha Avi-tag His-tag Protein, CF

For research use only

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