Recombinant Human IL-10 R alpha Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9044

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

Q13651.2

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IL-10 R alpha Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-10 R alpha protein
Human IL-10 R alpha
(His22-Asn235)
Accession # Q13651.2		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His22

Predicted Molecular Mass

53 kDa

SDS-PAGE

74-85 kDa, under reducing conditions

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.


Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-10 (Catalog # 217-IL/CF or Catalog # 217-ILB/CF) is coated at 2 µg/mL (100 μL/well), Biotinylated Recombinant Human IL‑10 R alpha Fc Chimera Avi-tag binds with an ED50 of 20-100 ng/mL

Measured by its ability to inhibit IL-10-dependent proliferation of MC/9-2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507.
The ED50 for this effect is 6‑48 ng/mL.

Scientific Data Images for Recombinant Human IL-10 R alpha Fc Avi-tag Protein, CF

Biotinylated Recombinant Human IL-10 R alpha Fc Chimera Avi-tag Protein Binding Activity.

When Recombinant Human IL-10 (217-ILB/CF) is coated at 2 μg/mL (100 μL/well), Biotinylated Recombinant Human IL-10 R alpha Fc Chimera Avi-tag (Catalog # AVI9044) binds with an ED50 of 20-100 ng/mL.

Biotinylated Recombinant Human IL-10 R alpha Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human IL-10 R alpha Fc Chimera Avi-tag (Catalog # AVI9044) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 74-85 kDa and 148-170 kDa.

Formulation, Preparation and Storage

AVI9044
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10 R alpha

Interleukin-10 Receptor alpha (IL-10 R alpha), also known as IL-10 R1, is a 90-110 kDa transmembrane glycoprotein member of the class II cytokine receptor family (1). IL-10 R alpha is required for mediating the effects of IL-10, a critical molecule in the control of microbial infections, allergic and autoimmune inflammation, and cancer (2-5). Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6). IL-10 R alpha is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL-10 R alpha, resulting in the recruitment of IL-10 R beta (6-8). IL-10 R beta is a ubiquitously expressed transmembrane protein that does not bind IL-10 by itself but is required for signal transduction and in vivo IL-10 responsiveness (7, 9). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (1). Immunosuppressive signal transduction through the IL-10 receptor complex can be inhibited by activation of TLR2, 4, or 9, enabling strengthened immune responses during infection (10). Polymorphisms of human IL-10 R alpha may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL-10 (11). Mature human IL-10 R alpha consists of a 214 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 322 aa cytoplasmic domain (12). Within the ECD, human IL-10 R alpha shares 59% aa sequence identity with mouse and rat IL-10 R alpha.

References

  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. Manzanillo, P. et al. (2015) Trends Immunol. 36:471.
  3. Sziksz, E. et al. (2015) Mediators Inflamm. 2015:764641.
  4. Mannino, M.H. et al. (2015) Cancer Lett. 367:103.
  5. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  6. Tan, J.C. et al. (1993) J. Biol. Chem. 268:21053.
  7. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  8. Tan, J.C. et al. (1995) J. Biol. Chem. 270:12906.
  9. Spencer, S.D. et al. (1998) J. Exp. Med. 187:571.
  10. Fernandez, S. et al. (2004) J. Immunol. 172:2613.
  11. Gruber, S.G. et al. (2008) Eur. J. Immunol. 38:3365.
  12. Liu, Y. et al. (1994) J. Immunol. 152:1821.

Long Name

Interleukin 10 Receptor alpha

Alternate Names

CDw210a, IL-10Ra, IL10R alpha, IL10RA

Entrez Gene IDs

3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)

Gene Symbol

IL10RA

UniProt

Q13651.2

Additional IL-10 R alpha Products

Product Documents for Recombinant Human IL-10 R alpha Fc Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human IL-10 R alpha Fc Avi-tag Protein, CF

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