Recombinant Human IL-10 R alpha Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 9044-RI
Key Product Details
Source
HEK293
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived human IL-10 R alpha protein
| Human IL-10 R alpha (His22-Asn235) Accession # Q13651 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
His22
Predicted Molecular Mass
51 kDa
SDS-PAGE
64-78 kDa, reducing conditions
Activity
Measured by its ability to inhibit IL-10-dependent proliferation of MC/9-2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507.
The ED50 for this effect is 6‑36 ng/mL
The ED50 for this effect is 6‑36 ng/mL
Formulation, Preparation and Storage
9044-RI
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-10 R alpha
(2-5). Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6). IL-10 R alpha is the ligand specific subunit of the IL-10 receptor complex. Noncovalent dimers of IL-10 bind to IL-10 R alpha, resulting in the recruitment of IL-10 R beta (6-8). IL-10 R beta is a ubiquitously expressed transmembrane protein that does not bind IL-10 by itself but is required for signal transduction and in vivo IL-10 responsiveness (7, 9). IL-10 R beta also associates with IL-20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (1). Immunosuppressive signal transduction through the IL-10 receptor complex can be inhibited by activation of TLR2, 4, or 9, enabling strengthened immune responses during infection (10). Polymorphisms of human IL-10 R alpha may limit viral immune evasion by retaining full responsiveness to human IL-10 but responding weakly to the cytomegalovirus homolog of IL-10 (11). Mature human IL-10 R alpha consists of a 214 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 322 aa cytoplasmic domain (12). Within the ECD, human IL-10 R alpha shares 59% aa sequence identity with mouse and rat IL-10 R alpha.
References
- Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
- Manzanillo, P. et al. (2015) Trends Immunol. 36:471.
- Sziksz, E. et al. (2015) Mediators Inflamm. 2015:764641.
- Mannino, M.H. et al. (2015) Cancer Lett. 367:103.
- Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
- Tan, J.C. et al. (1993) J. Biol. Chem. 268:21053.
- Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
- Tan, J.C. et al. (1995) J. Biol. Chem. 270:12906.
- Spencer, S.D. et al. (1998) J. Exp. Med. 187:571.
- Fernandez, S. et al. (2004) J. Immunol. 172:2613.
- Gruber, S.G. et al. (2008) Eur. J. Immunol. 38:3365.
- Liu, Y. et al. (1994) J. Immunol. 152:1821.
Long Name
Interleukin 10 Receptor alpha
Alternate Names
CDw210a, IL-10Ra, IL10R alpha, IL10RA
Gene Symbol
IL10RA
UniProt
Additional IL-10 R alpha Products
Product Documents for Recombinant Human IL-10 R alpha Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human IL-10 R alpha Fc Chimera Protein, CF
For research use only
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