Recombinant Human IL-10 R beta Fc Chimera Avi Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11460

Avi-tag
R&D Systems, part of Bio-Techne
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AVI11460-050
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Key Product Details

Source

CHO

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IL-10 R beta Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-10 R beta protein
Human IL-10RB
(Met20-Ser220)
Accession # Q08334.2		 GGIEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met20

Predicted Molecular Mass

52 kDa

SDS-PAGE

78-88 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human IL-10 R beta Fc Chimera Avi-tag (Catalog # AVI11460) binds Recombinant Human IL-28 R alpha/IFN-lambda R1 Fc Chimera (Catalog # 5260-MR) in the presence of Recombinant Human IL-28B/IFN-lambda 3 (Catalog # 5259-IL) with an ED50 of 50.0-750 ng/mL.

Scientific Data Images for Recombinant Human IL-10 R beta Fc Chimera Avi Protein, CF

Biotinylated Recombinant Human IL-10 R beta Fc Chimera Avi-tag Protein Binding Activity.

Biotinylated Recombinant Human IL-10 R beta Fc Chimera Avi-tag (Catalog # AVI11460) binds Recombinant Human IL-28 R alpha/IFN-lambda R1 Fc Chimera (5260-MR) in the presence of Recombinant Human IL-28B/IFN-lambda 3 (5259-IL) with an ED50 of 50.0-750 ng/mL.

Biotinylated Recombinant Human IL-10 R beta Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human IL-10 R beta Fc Chimera Avi-tag Protein (Catalog # AVI11460) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 78-88 kDa and 160-170 kDa, respectively.

Formulation, Preparation and Storage

AVI11460
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in sterile water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10 R beta

Interleukin-10 Receptor beta (IL-10 R beta), also known as IL-10 R2 and CRF2-4, is a 60 kDa transmembrane glycoprotein that functions as a co-receptor for several class 2 cytokines including Interleukins-10, -22, -26, -28A/IFN-lambda 2, -28B/IFN-lambda 3, and -29/IFN-lambda (1, 2). IL-10 R beta associates with ligand‑specific receptor subunits to form signaling receptor complexes, e.g. IL-10 R alpha for IL-10 (3, 4), IL-20 R alpha for IL-26 (5, 6), IL-22 R alpha for IL-22 (7, 8), and IL-28 R alpha for IL-28A, IL-28B, and IL-29 (9, 10). IL‑10 R beta is widely expressed, while the associated receptor subunits exhibit differential expression patterns (1). The ligand‑specific subunits are responsible for the divergent functions of these cytokines, encompassing immune suppression, promotion or inhibition of inflammation, mucosal defense, antiviral immunity, and hematopoiesis (1). IL-10 R beta deficient mice lack responsiveness to each of those cytokines. IL-10 R beta contributes to ligand binding, but effective signaling is only triggered in the presence of the ligand‑specific subunit (8, 9, 11). In the case of IL-10, a cytokine dimer binds to two IL‑10 R alpha/IL-10R1 chains, resulting in recruitment of two IL-10 R beta/IL-10R2 chains (3, 12). Some members of the IL-10 family are monomeric cytokines and interact with single molecules of IL-10 R beta and their ligand‑specific subunit (1). Mature human IL-10 R beta consists of a 201 amino acid (aa) extracellular region with two fibronectin type-III domains, a 22 aa transmembrane segment and a 83 aa cytoplasmic domain (13). Within the ECD, human IL-10 R beta shares 75% and 78% aa sequence identity with mouse and rat IL-10 R beta, respectively. Our Avi-tag Biotinylated human IL-10 R beta biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Commins, S. et al. (2008) J. Allergy Clin. Immunol. 121:1108.
  2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  3. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  4. Spencer, S.D. et al. (1998) J. Exp. Med. 187:571.
  5. Sheikh, F. et al. (2004) J. Immunol. 172:2006.
  6. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
  7. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  8. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
  9. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
  10. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  11. Yoon, S.I. et al. (2006) J. Biol. Chem. 281:35088.
  12. Pletnev, S. et al. (2005) BMC Struct. Biol. 5:10.
  13. Lutfalla, G. et al. (1993) Genomics 16:366.

Long Name

Interleukin 10 Receptor beta

Alternate Names

CDw210b, CRFB4, D21S58, IL-10R2, IL-10Rb, IL10R beta, IL10RB

Entrez Gene IDs

3588 (Human); 16155 (Mouse)

Gene Symbol

IL10RB

Additional IL-10 R beta Products

Product Documents for Recombinant Human IL-10 R beta Fc Chimera Avi Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-10 R beta Fc Chimera Avi Protein, CF

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