Recombinant Human IL-10 R beta Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 874-RB

R&D Systems, part of Bio-Techne
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874-RB-100
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Key Product Details

Source

NS0

Accession #

Q08334

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-10 R beta Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IL-10 R beta protein
Human IL-10 R beta
(Met20-Ser220)
Accession # Q08334		 DIEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met20

Predicted Molecular Mass

51 kDa (monomer)

SDS-PAGE

75-85 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL 28 R alpha /IFN lambda R1 Fc Chimera (Catalog # 5260-MR) is immobilized at 5.00 μg/mL (100 μL/well), the concentration of Recombinant Human IL-10 R beta Fc Chimera that produces 50% of the optimal binding response is found to be approximately 0.300-3.00 μg/mL.

Formulation, Preparation and Storage

874-RB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10 R beta

Interleukin-10 Receptor beta (IL-10 R beta), also known as IL-10 R2 and CRF2-4, is a 60 kDa transmembrane glycoprotein that functions as a co-receptor for several class 2 cytokines including Interleukins-10, -22, -26, -28A/IFN-lambda 2, -28B/IFN-lambda 3, and -29/IFN-lambda (1, 2). IL-10 R beta associates with ligand-specific receptor subunits to form signaling receptor complexes, e.g. IL-10 R alpha for IL-10 (3, 4), IL-20 R alpha for IL-26 (5, 6), IL-22 R alpha for IL-22 (7, 8), and IL-28 R alpha for IL-28A, IL-28B, and IL-29 (9, 10). IL-10 R beta is widely expressed, while the associated receptor subunits exhibit differential expression patterns (1). The ligand-specific subunits are responsible for the divergent functions of these cytokines, encompassing immune suppression, promotion or inhibition of inflammation, mucosal defense, antiviral immunity, and hematopoiesis (1). IL-10 R beta deficient mice lack responsiveness to each of those cytokines. IL-10 R beta contributes to ligand binding, but effective signaling is only triggered in the presence of the ligand-specific subunit (8, 9, 11). In the case of IL-10, a cytokine dimer binds to two IL‑10 R alpha/IL-10R1 chains, resulting in recruitment of two IL-10 R beta/IL-10R2 chains (3, 12). Some members of the IL-10 family are monomeric cytokines and interact with single molecules of IL-10 R beta and their ligand-specific subunit (1). Mature human IL-10 R beta consists of a 201 amino acid (aa) extracellular region with two fibronectin type-III domains, a 22 aa transmembrane segment and a 83 aa cytoplasmic domain (13). Within the ECD, human IL-10 R beta shares 75% and 78% aa sequence identity with mouse and rat IL-10 R beta, respectively.

References

  1. Commins, S. et al. (2008) J. Allergy Clin. Immunol. 121:1108.
  2. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  3. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  4. Spencer, S.D. et al. (1998) J. Exp. Med. 187:571.
  5. Sheikh, F. et al. (2004) J. Immunol. 172:2006.
  6. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
  7. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  8. Xie, M.-H. et al. (2000) J. Biol. Chem. 275:31335.
  9. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
  10. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  11. Yoon, S.I. et al. (2006) J. Biol. Chem. 281:35088.
  12. Pletnev, S. et al. (2005) BMC Struct. Biol. 5:10.
  13. Lutfalla, G. et al. (1993) Genomics 16:366.

Long Name

Interleukin 10 Receptor beta

Alternate Names

CDw210b, CRFB4, D21S58, IL-10R2, IL-10Rb, IL10R beta, IL10RB

Entrez Gene IDs

3588 (Human); 16155 (Mouse)

Gene Symbol

IL10RB

UniProt

Q08334

Additional IL-10 R beta Products

Product Documents for Recombinant Human IL-10 R beta Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-10 R beta Fc Chimera Protein, CF

For research use only

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