Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10382

R&D Systems, part of Bio-Techne
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AVI10382-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P42701-1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all IL-12 R beta 1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-12 R beta 1 protein
Human IL-12 R beta1
(Cys24-Glu540)
Accession # P42701-1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

85 kDa

SDS-PAGE

95-115 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human IL-12 (Catalog # 219-IL) is immobilized at 1 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human IL-12 beta1 Fc Chimera Avi-tag (Catalog # AVI10382) that produces 50% of the optimal binding response is found to be approximately 15-90 ng/mL.

Scientific Data Images for Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein, CF

Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein Binding Activity

Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein Binding Activity

When Recombinant Human IL-12 (Catalog # 219-IL) is immobilized at 1 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human IL-12 beta1 Fc Chimera Avi-tag (Catalog # AVI10382) that produces 50% of the optimal binding response if found to be approximately 15-90 ng/mL.
Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein SDS-PAGE

Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human IL-12 R beta1 Fc Chimera Avi-tag (Catalog # AVI10382) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 95-115 kDa and 180-230 kDa, respectively.

Formulation, Preparation and Storage

AVI10382
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12 R beta 1

IL12RB1 (Interleukin-12 receptor subunit beta-1; IL‑12 R beta1), also known as CD212, is a type I transmembrane protein with similarities with the gp130/G-CSF R subgroup in the cytokine receptor superfamily. IL12RB1, along with IL12RB2, are two receptor subunits for IL12. Individually, the two subunits bind IL12 with low affinity, but when combined they form a high-affinity receptor complex (1). Human IL12RB1 contains 5 fibronectin domains in the extracellular domain (ECD), a single transmembrane domain and a box 1 motif in the cytoplasmic region. The mature ECD of human IL12RB1 shares 51% amino acid identity with mouse and rat IL12RB1. Interleukin 12 (IL12) is a pro-inflammatory cytokine and key mediator of cellular-immunity and induces the differentiation of Th1 cells from precursor T helper cells (2). Binding of IL12RB1, along with IL12RB2, is required for IL12 signal transduction and subsequent activity (1,2). Expression of IL12RB1 been detected in activated T cells, NK cells and B cells (1). The expression of IL12RB2 is more restricted and appears to be limited to Th2 cells. IL12RB1 has been reported to interact with IL23R to form the functional receptor complex for IL23 and probably activation of the Jak-Stat signaling cascade (3).

References

  1. Robinson R.T. (2015) Cytokine. 71:348.
  2. Rosenzweig S.D. and Holland S.M. (2005) Immunol. Rev. 203:38.
  3. Zou J. et al. (1997) J. Biol Chem, 272:6073.

Long Name

Interleukin 12 Receptor beta 1

Alternate Names

CD212, IL-12Rb1, IL12R beta 1, IL12RB1

Entrez Gene IDs

3594 (Human); 16161 (Mouse)

Gene Symbol

IL12RB1

UniProt

P42701-1

Additional IL-12 R beta 1 Products

Product Documents for Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human IL-12 R beta 1 Fc Avi-tag Protein, CF

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