Recombinant Human IL-13 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 213-ILB/CF

R&D Systems, part of Bio-Techne
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213-ILB-01MG/CF
213-ILB-025/CF
213-ILB-100/CF
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Conjugate
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Unconjugated
213-ILB
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Key Product Details

Source

E. coli

Accession #

AAK53823

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-13 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-13 protein
Gly21-Asn132

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly21

Predicted Molecular Mass

12.3 kDa

SDS-PAGE

9 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 0.5-2.5 ng/mL.

Scientific Data Images for Recombinant Human IL-13 Protein, CF

Graph showing dose-dependent bioactivity of Human IL-13 protein

Bioactivity of Human IL-13 Protein

Recombinant human IL-13 (213-ILB/CF) stimulates proliferation in the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.5-2.5 ng/mL.

Formulation, Preparation and Storage

213-ILB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-13

Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells (1‑3). Mature human IL-13 shares approximately 58% amino acid sequence identity with mouse and rat IL-13. Despite the low homology, it exhibits cross‑species activity between human, mouse, and rat (4). IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. On B cells, it promotes cellular activation, immunoglobulin class switching to IgE, and the up‑regulation of CD23/Fc epsilon RII (1, 5). IL-13 binds with low affinity to the transmembrane IL-13 R alpha1 which then forms a signaling complex with the transmembrane IL-4 R alpha (6‑8). This high affinity receptor complex also functions as the type 2 IL-4 receptor (6, 7). IL‑13 R alpha2 inhibits responses to both IL-13 and IL-4. It binds IL-13 with high affinity (9, 10) and prevents IL-13 from signaling through the IL-13 R alpha1/IL-4 R alpha complex (11, 12). It also blocks signaling through IL-4-occupied IL-13 R alpha1/IL-4 R alpha receptor complexes (12, 13). In addition, IL-13-bound IL-13 R alpha2 can directly promote tumor cell invasiveness and the development of tissue fibrosis (14‑16).

References

  1. Saggini, A. et al. (2011) Int. J. Immunopathol. Pharmacol. 24:305.
  2. Gallo, E. et al. (2012) Eur. J. Immunol. 42:2322.
  3. Liang, H.E. et al. (2012) Nat. Immunol. 13:58.
  4. Ruetten, H. and C. Thiemermann (1997) Shock 8:409.
  5. McKenzie, A.N. et al. (1993) Proc. Natl. Acad. Sci. USA 90:3735.
  6. Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
  7. Zurawski, S.M. et al. (1995) J. Biol. Chem. 270:13869.
  8. Andrews, A.L. et al. (2002) J. Biol. Chem. 277:46073.
  9. Chen, W. et al. (2009) J. Immunol. 183:7870.
  10. Daines, M.O. et al. (2007) J. Allergy Clin. Immunol. 119:375.
  11. Kasaian, M.T. et al. (2011) J. Immunol. 187:561.
  12. Andrews, A.-L. et al. (2006) J. Allergy Clin. Immunol. 118:858.
  13. Rahaman, S.O. et al. (2002) Cancer Res. 62:1103.
  14. Fujisawa, T. et al. (2009) Cancer Res. 69:8678.
  15. Fujisawa, T. et al. (2011) Int. J. Cancer 131:344.
  16. Fichtner-Feigl, S. et al. (2006) Nat. Med. 12:99.

Long Name

Interleukin 13

Alternate Names

IL13, NC30, P600

Entrez Gene IDs

3596 (Human); 16163 (Mouse); 116553 (Rat); 574325 (Primate)

Gene Symbol

IL13

UniProt

AAK53823

Additional IL-13 Products

Product Documents for Recombinant Human IL-13 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-13 Protein, CF

For research use only

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