Recombinant Human IL-13 R alpha 1 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 146-IR
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Binding Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human IL-13 R alpha 1 protein
| Human IL-13 R alpha1 Ala27 - Thr343 (Thr130Ile) Accession # Q5JSL4 |
IEGRMD | Human IgG1 (Pro100 - Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala27
Predicted Molecular Mass
64 kDa (monomer)
SDS-PAGE
110 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
Immobilized rhIL-13 R alpha1/Fc Chimera at 4 µg/mL (100 µL/well) can bind rhIL-13 with a linear range of 1-100 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Immobilized rhIL-13 R alpha1/Fc Chimera at 4 µg/mL (100 µL/well) can bind rhIL-13 with a linear range of 1-100 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Formulation, Preparation and Storage
146-IR
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-13 R alpha 1
Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha' or NR4, is now referred to as IL-13 R alpha1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha', is now referred to as IL-13 R alpha2.
The human IL-13 R alpha1 was originally cloned based on sequence homology to the mouse IL-13 R alpha1. The IL-13 R alpha1 cDNA encodes a 427 amino acid (aa) residue precursor protein with a putative 21 aa residue signal peptide, a 324 aa residue extracellular domain, a 23 aa residue transmembrane region and a 59 aa residue cytoplasmic tail. Human and mouseIL-13 R alpha1 share 76% aa sequence identity. The extracellular domain of IL-13 R alpha1 is also closely related to that of IL-13 R alpha2. IL-13 R alpha1 has been shown to combine with the IL-4 R alpha to form a high-affinity receptor complex capable of transducing an IL-13-dependent proliferative signal. The role of IL-13 R alpha2 in IL-13 signaling remains to be elucidated.
References
- Caput, D. et al. (1996) J. Biol. Chem. 271:16921.
- Donaldson, D.D. et al. (1998) J. Immunol. 161:2317.
- Aman, M.J. et al. (1996) J. Biol. Chem. 271:29265.
- Hilton, D.J. et al. (1996) Proc. Natl. Acad. Sci. USA 93:497.
- Zhang, J.G. et al. (1997) J. Biol. Chem. 272:9474.
Long Name
Interleukin 13 Receptor alpha 1
Alternate Names
CD213a1, IL-13Ra1, IL13R alpha 1, IL13RA1
Gene Symbol
IL13RA1
UniProt
Additional IL-13 R alpha 1 Products
Product Documents for Recombinant Human IL-13 R alpha 1 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human IL-13 R alpha 1 Fc Chimera Protein, CF
For research use only
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