Recombinant Human IL-13 R alpha 1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 11424-IR

R&D Systems, part of Bio-Techne
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11424-IR-100
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Key Product Details

Source

HEK293

Accession #

P78552.1

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-13 R alpha 1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-13 R alpha 1 protein
Ala27-Thr343, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala27

Predicted Molecular Mass

37 kDa

SDS-PAGE

58-64 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human IL-13 R alpha1 His-tag (Catalog # 11424-IR) binds Recombinant Human IL-13 (Catalog # 213-ILB/CF) with an ED50 of 37.5-450 ng/mL.

Scientific Data Images for Recombinant Human IL-13 R alpha 1 His-tag Protein, CF

Recombinant Human IL‑13 R alpha1 His-tag Protein Binding Activity.

Recombinant Human IL‑13 R alpha1 His-tag Protein (Catalog # 11424-IR) binds Recombinant Human IL-13 (213-ILB/CF) with an ED50 of 37.5-450 ng/mL.

Recombinant Human IL‑13 R alpha1 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL‑13 R alpha1 His-tag Protein (Catalog # 11424-IR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 58-64 kDa, under reducing conditions.

Formulation, Preparation and Storage

11424-IR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-13 R alpha 1

IL-13 RA1, also known as IL-13R and IL-13 RA, is a type I transmembrane protein. Its cDNA encodes a 427 aa precursor protein, with 322 aa extracellular domain, 24 aa transmembrane domain and 60 aa intracellular domain. Within the extracellular domain, human IL-13 RA1 shares 75% and 74% homology with mouse and rat IL-13 RA1, respectively. IL-13 RA1 expresses ubiquitously in all tissues with the highest level in heart, liver, skeletal muscle and ovary (1). As a receptor, IL-13 RA1 can function alone or as a heterodimer with IL-4R. Although both IL-4 and IL-13 signal through IL-4R/IL-13 RA1 heterodimer, there are distinct differences. IL-4 binds IL-4R with high affinity then binds IL-13 RA1 with low affinity. In contrast, IL-13 binds IL-13 RA1 with decent affinity, then binds IL-4R with high affinity (2). In addition, the N-terminal Fibronectin type III domain (D1) of IL-13 RA1 is only required for the binding of IL-13 not IL-4 (3,4). After binding to IL-4 or IL-13, the Tyr residues in the cytoplasmic domain of IL-13 RA get phosphorylated and then activate signaling proteins including Jak1, Tyk1, Tyk2, IRS-1, and STAT6 (5, 6). Alternative splicing generates soluble iL-13 RA1 missing the transmembrane domain (7). It not only functions as a decoy receptor for IL-13, but also is able to reduce fasting blood glucose, mediated by IL-4 (8). Higher expression of IL-13 RA1 are found in several cancers, often associated with poor prognosis in patients (9-11). 

References

  1. Aman M.J. et al. (1996) J. Biol. Chem. 271:29265.
  2. LaPorte S.L. et al. (2008) Cell 132:259.
  3. Arima K. et al. (2005) J. Biol. Chem. 280:24915.
  4. Ito T, et al. (2009) J. Biol. Chem. 284:24289.
  5. Umeshita-Suyama R. et al. (2000) Int. Immunol. 12:1499.
  6. Roy B, et al. (2002) J. Leukoc. Biol. 72:580.
  7. Osawa M, et al. (2000) Immunogenetics 51:974.
  8. Rachmin I, et al. (2017) Am. J. Physiol. Endocrinol. Metab. 313:E663.
  9. Park M.H. et al. (2017) Ann, Surg. Oncol. 24:3780.
  10. Cao H, et al. (2016) Oncotarget 7:61183.
  11. Suzuki A, et al. (2015) Cytokine 75:79.

Long Name

Interleukin 13 Receptor alpha 1

Alternate Names

CD213a1, IL-13Ra1, IL13R alpha 1, IL13RA1

Entrez Gene IDs

3597 (Human); 16164 (Mouse)

Gene Symbol

IL13RA1

UniProt

P78552.1

Additional IL-13 R alpha 1 Products

Product Documents for Recombinant Human IL-13 R alpha 1 His-tag Protein, CF

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Product Specific Notices for Recombinant Human IL-13 R alpha 1 His-tag Protein, CF

For research use only

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