Recombinant Human IL-15R alpha Fc Chimera Avi-tag, CF

R&D Systems, part of Bio-Techne | Catalog # AVI7194

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

Q13261.1

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Binding Activity, Bioactivity

View all IL-15R alpha Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human IL-15R alpha protein
Human IL-15R alpha
(Ile31-Thr205)
Accession # Q13261.1		 IEGRMD
 Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile31

Predicted Molecular Mass

47 kDa

SDS-PAGE

70-80 kDa, under reducing conditions

Activity

The biotin to protein ratio is greater than 0.7 as determined by the HABA assay.

Measured by its binding ability in a functional ELISA.
When Biotinylated Recombinant Human IL-15R alpha Fc Chimera Avi-tag (Catalog # AVI7194) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human IL-15  (Catalog # 247-ILB) binds with an ED50 of 0.02-0.16 ng/mL.

Scientific Data Images for Recombinant Human IL-15R alpha Fc Chimera Avi-tag, CF

Biotinylated Recombinant Human IL-15R alpha Fc Chimera Avi-tag Protein Binding Activity.

When Biotinylated Recombinant Human IL-15R alpha Fc Chimera Avi-tag (Catalog # AVI7194) is immobilized at 0.5 µg/mL (100 µL/well), Recombinant Human IL-15 (247-ILB) binds with an ED50 of 0.02-0.16 ng/mL.

Biotinylated Recombinant Human IL-15R alpha Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human IL-15R alpha Fc Chimera Avi-tag (Catalog # AVI7194) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 70-80 kDa and 140-160 kDa, respectively.

Formulation, Preparation and Storage

AVI7194
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-15R alpha

Interleukin 15 Receptor alpha (IL‑15 R alpha ), also known as CD215, is a widely expressed 60-kDa transmembrane glycoprotein that plays an important role in the homeostasis and activation of NK cells and CD8+ memory T cells and participates in the development and function of many other hematopoietic cell types and non‑immune cell types (1‑3). Mature human IL‑15 R alpha consists of a 175 amino acid (aa) extracellular domain (ECD) containing one N‑linked glycosylation site, a 23 aa transmembrane segment, and a 39 aa cytoplasmic tail (4). Within the ECD, human IL‑15 R alpha shares approximately 60% aa sequence identity with mouse and rat IL‑15 R alpha. Alternate splicing of human IL‑15 R alpha generates additional isoforms with variable length deletions in the ECD and/or substitutions in the cytoplasmic domain (4, 5). IL‑15 R alpha binds to Interleukin‑15 with high affinity (6). IL‑15 additionally interacts with lower affinity to a complex of IL‑2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL‑2 receptor complex (7, 8). The use of shared receptor components contributes to the overlapping biological effects of IL‑15 and IL‑2. The dominant mechanism of IL‑15 action is known as transpresentation in which IL‑15/IL‑15 R alpha complexes are expressed on the surface of one cell and interact with complexes of IL‑2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL‑15 even if they do not express IL-15 R alpha (10‑12). IL‑15/IL‑15 R alpha complexes can transmit reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL‑15/IL‑15 R alpha expressing cells (13, 14). Shed soluble forms of IL‑15 R alpha retain the ability to bind tightly to IL‑15 and can inhibit IL‑15 bioactivity (6, 15, 16). Our Avi-tag Biotinylated Recombinant Human IL‑15 R alpha features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  2. Di Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
  3. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
  4. Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
  5. Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.
  6. Giri, J.G. et al. (1995) EMBO 14:3654.
  7. Grabstein, K. et al. (1994) Science 264:965.
  8. Giri, J. et al. (1994) EMBO J. 13:2822.
  9. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
  10. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
  11. Dubois, S. et al. (2002) Immunity 17:537.
  12. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
  13. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  14. Neely, G.G. et al. (2004) J. Immunol. 172:4225.
  15. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  16. Mortier, E. et al. (2004) J. Immunol. 173:1681.

Long Name

Interleukin 15 Receptor alpha

Alternate Names

CD215, IL-15 R alpha, IL-15Ra, IL15 R alpha, IL15RA

Entrez Gene IDs

3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)

Gene Symbol

IL15RA

UniProt

Q13261.1

Additional IL-15R alpha Products

Product Documents for Recombinant Human IL-15R alpha Fc Chimera Avi-tag, CF

Product Datasheets

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Product Specific Notices for Recombinant Human IL-15R alpha Fc Chimera Avi-tag, CF

For research use only

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