Recombinant Human IL-17A Biotinylated Protein, CF

R&D Systems, part of Bio-Techne | Catalog # BT7955B

Biotinylated
R&D Systems, part of Bio-Techne
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BT7955B-025
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Key Product Details

Source

HEK293

Accession #

Q16552.1

Structure / Form

Disulfide-linked homodimer
Biotinylated via amines

Conjugate

Biotin

Applications

Bioactivity

View all IL-17/IL-17A Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human IL-17/IL-17A protein
Gly24-Ala155

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

15.1 kDa

SDS-PAGE

12-25 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human IL‑17/IL‑17A (Catalog # BT7955B) binds to Recombinant Human IL-17RA/IL-17R Fc Chimera Protein (Catalog # 177-IR) with a ED50 of 2.00-20.0 ng/mL.

Scientific Data Images for Recombinant Human IL-17A Biotinylated Protein, CF

Biotinylated Recombinant Human IL‑17/IL‑17A Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human IL‑17/IL‑17A (Catalog # BT7955B) binds to Recombinant Human IL-17RA/IL-17R Fc Chimera Protein (177-IR) with a ED50 of 2.00-20.0 ng/mL.

Formulation, Preparation and Storage

BT7955B
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with Trehalose.
Reconstitution Reconstitute at 100 µg/mL in sterile 4 mM HCL.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17/IL-17A

Interleukin-17A (IL-17A), also known as CTLA-8, is a 15-20 kDa glycosylated cytokine that plays an important role in anti-microbial and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold (1, 2). Mature human IL-17A shares 60% amino acid sequence identity with mouse and rat IL-17A (3, 4). IL-17A is secreted by Th17 cells, gamma/ delta T cells, iNKT cells, NK cells, LTi cells, neutrophils, and intestinal Paneth cells (2). It forms disulfide-linked homodimers as well as disulfide-linked heterodimers with IL-17F (5, 6). IL-17A exerts its effects through the transmembrane IL-17RA in complex with IL-17RC or IL-17RD (7, 8). Both IL-17RA and IL-17RC are required for responsiveness to heterodimeric IL-17A/F (7). IL-17A promotes protective mucosal and epidermal inflammation in response to microbial infection (9-12). It induces chemokine production, neutrophil influx, and the production of antibacterial peptides
(9-11). IL-17A/F likewise induces neutrophil migration, but IL-17F does not (11). IL-17A additionally enhances the production of inflammatory mediators by rheumatoid synovial fibroblasts and contributes to TNF-alpha induced shock (4, 13). In contrast, it can protect against the progression of colitis by limiting chronic inflammation (12). IL-17A encourages the formation of autoreactive germinal centers and exacerbates the onset and progression of experimental models of autoimmunity (14, 15).
IL-17A has been shown to exert either tumorigenic or anti-tumor effects (16, 17).

References

  1. Gaffen, S.L. (2009) Nat. Rev. Immunol. 9:556.
  2. Cua, D.J. and C.M. Tato (2010) Nat. Rev. Immunol. 10:479.
  3. Yao, Z. et al. (1995) J. Immunol. 155:5483.
  4. Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.
  5. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
  6. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  7. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
  8. Rong, Z. et al. (2009) Cell Res. 19:208.
  9. Cho, J.S. et al. (2010) J. Clin. Invest. 120:1762.
  10. Liang, S.C. et al. (2006) J. Exp. Med. 203:2271.
  11. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
  12. O’Connor Jr., W. et al. (2009) Nat. Immunol. 10:603.
  13. Takahashi, N. et al. (2008) J. Exp. Med. 205:1755.
  14. Hsu, H. et al. (2008) Nat. Immunol. 9:166.
  15. Rohn, T.A. et al. (2006) Eur. J. Immunol. 36:2857.
  16. Wang, L. et al. (2009) J. Exp. Med. 206:1457.
  17. Kryczek, I. et al. (2009) Blood 114:357.

Long Name

Interleukin 17

Alternate Names

CTLA-8, CTLA8, IL-17A, IL17, IL17A

Entrez Gene IDs

3605 (Human); 16171 (Mouse); 301289 (Rat); 449530 (Porcine); 481837 (Canine); 102119976 (Cynomolgus Monkey)

Gene Symbol

IL17A

UniProt

Q16552.1

Additional IL-17/IL-17A Products

Product Documents for Recombinant Human IL-17A Biotinylated Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human IL-17A Biotinylated Protein, CF

For research use only

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